紫荆花花色苷提取工艺优化及表征  

Extraction process optimization of anthocyanins from Cercis chinensis flower and their structure characterization

作　　者：王收 张傲涵 李亚菲 张林 潘丽[1] 张玉焕 贾紫尧 杨揆 李雪琴[1] 谷克仁[1] WANG Shou;ZHANG Aohan;LI Yafei;ZHANG Lin;PAN Li;ZHANG Yuhuan;JIA Ziyao;YANG Kui;LI Xueqin;GU Keren(Grain,Oil and Food Engineering Technology Research Center of the State Grain and Reserves Administration/Key Laboratory of Henan Province,Henan University of Technology,Zhengzhou 450001,China;Yanling Cercis Biotechnology Co.,Ltd,Xuchang 461200,China;Yanling Zhonglin Garden Engineering Co.,Ltd,Xuchang 461200,China)

机构地区：[1]河南工业大学国家粮食和物资储备局粮油食品工程技术研究中心/河南省重点实验室,河南郑州450001 [2]鄢陵县紫荆生物科技有限公司,河南许昌461200 [3]鄢陵中林园林工程有限公司,河南许昌461200

出　　处：《河南工业大学学报（自然科学版）》2024年第5期64-71,130,共9页Journal of Henan University of Technology：Natural Science Edition

基　　金：国家自然科学基金青年科学基金项目(32001738);郑州市科技局自然科学项目(21ZZXTCX18);省部级科研平台开放课题(GO202209);河南工业大学青年骨干教师培育计划(20201101)。

摘　　要：紫荆花的花色苷含量十分丰富,且具有良好的生物活性。为了高效提取紫荆花花色苷,充分发挥其应用价值,以紫荆花为原料、乙醇为提取剂,采用超声辅助法提取花色苷,通过单因素和响应面试验确定花色苷的最佳提取条件,使用傅里叶红外光谱技术及超高效液相色谱串联质谱技术对紫荆花花色苷结构进行了表征。结果表明:紫荆花花色苷最佳提取工艺条件为液料比33∶1 mL/g、乙醇体积分数77%、超声功率326 W、超声时间15 min、超声温度40℃,在此条件下,紫荆花花色苷的含量为(3.40±0.06)mg/g;紫荆花花色苷分子结构中含有羟基、含氧环、糖基、苯环和甲氧基;共得到17种花色苷,3种花色苷衍生物。该研究为紫荆花花色苷在食品工业中的应用提供了理论依据。The extract of Cercis chinensis flowers is rich in anthocyanins and possesses good biological activity.In order to efficiently extract the anthocyanins from Cercis chinensis flowers and fully realize their application value,using flowers as the raw material and ethanol as the extractant an ultrasonic-assisted method was adopted to extract the anthocyanins.The extraction conditions were determined through single-factor experiments and response surface optimization.The results showed that the optimal conditions were a liquid-to-solid ratio of 33∶1 mL/g,an ethanol volume fraction of 77%,an ultrasonic power of 326 W,an ultrasonic time of 15 min,and an ultrasonic temperature of 40℃.Under these conditions,the content of Cercis chinensis flower anthocyanins was(3.40±0.06)mg/g,which is close to the predicted value.Fourier transform infrared spectroscopy was used to analyze the Cercis chinensis flower anthocyanins,indicating that the molecular structure of the anthocyanins contains hydroxyl groups,oxygen rings,glycosides,phenyl rings,and methoxy groups.In addition,ultra-high-performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS)was used to identify the components of the Cercis chinensis flower anthocyanins,resulting in the identification of 17 types of anthocyanins and 3 anthocyanin derivatives.The optimal extraction conditions for Cercis chinensis flower anthocyanins were obtained through this research,and the composition of the Cercis chinensis flower anthocyanins was studied,providing a theoretical basis for their application in the food industry.

关 键 词：紫荆花 花色苷 超声辅助法 响应面试验 表征 

分 类 号：TS201.2[轻工技术与工程—食品科学]