三种稻飞虱Toll受体基因家族比较分析  

Comparative Analysis of the Toll Receptor Gene Families in Three Species of Rice Planthoppers

作　　者：钟梓春 吴洪鑫 张杰[1] 郭玉静 何柳燕 许小霞[1] 金丰良[1] 庞锐 ZHONG ZiChun;WU HongXin;ZHANG Jie;GUO YuJing;HE LiuYan;XU XiaoXia;JIN FengLiang;PANG Rui(College of Plant Protection,South China Agricultural University/State Key Laboratory of Green Pesticide,Guangzhou 510642)

机构地区：[1]华南农业大学植物保护学院/绿色农药全国重点实验室,广州510642

出　　处：《中国农业科学》2024年第20期4007-4021,共15页Scientia Agricultura Sinica

基　　金：国家自然科学基金(32172498)。

摘　　要：【目的】Toll受体(Toll receptor)是昆虫先天免疫系统中Toll信号通路中的关键效应因子之一。本文旨在鉴定3种稻飞虱(褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera、灰飞虱Laodelphax striatellus)的Toll受体基因,探索Toll受体在3种稻飞虱中的潜在功能及其种间差异,为稻飞虱免疫发育研究以及稻飞虱的防控提供理论依据。【方法】利用生物信息学方法从3种稻飞虱的基因组中鉴定出Toll受体基因,分析其基因结构和特征、编码蛋白的理化性质及其结构域、染色体定位及系统发育进化关系。利用人工智能软件AlphaFold 3预测Toll受体的三维结构,并与目前已知结构和功能的其他物种Toll受体进行比较,预测其潜在功能及种间功能分化。通过转录组数据定量分析Toll受体基因在不同组织和不同发育阶段的表达情况。【结果】分别在褐飞虱、白背飞虱和灰飞虱基因组中鉴定出6、7和6个Toll受体基因,均分布在1、4、7号染色体上,且分布规律明显,3种稻飞虱的Toll受体基因家族均在1号、4号染色体上分布一个基因,其余分布在7号染色体上。3种稻飞虱Toll受体基因编码序列长度为2676—4158 bp,外显子数量1—7个,编码蛋白序列长度891—1385 aa,分子量大小为103.31—158.25 kDa,理论等电点范围为5.42—6.54。系统发育分析表明,3种稻飞虱的Toll受体基因家族可以分为6个亚家族,分别与其他昆虫的Toll、Toll6、Toll7、Tollo(Toll8)以及Toll9同源。使用AlphaFold 3预测得到的胞外结构与其他物种Toll受体胞外结构比较分析结果显示,在白背飞虱的Toll受体基因家族中预测分析出2个Toll受体与病毒互作潜在相关,在灰飞虱中有1个Toll受体与病毒互作潜在相关,而在褐飞虱中没有匹配出相关的Toll受体。转录组定量结果表明,3种稻飞虱的Toll受体基因在不同组织和不同发育阶段间均有表达,推测其可能拥有不同功能,参与不�【Objective】The Toll receptor is one of the key effector factors in the Toll signaling pathway of the innate immune system in insects.This article aims to identify the Toll receptor genes of three types of rice planthoppers(Nilaparvata lugens,Sogatella furcifera,and Laodelphax striatellus),explore the potential functions of the Toll receptor in these three species,and investigate the interspecies differences,so as to provide a theoretical basis for the study of the immune development of rice planthoppers and for the control and prevention of these pests.【Method】Bioinformatics methods were used to identify Toll receptor genes from the genomes of three species of rice planthoppers,and the gene structure and characteristics,physicochemical properties and structural domains of the encoded proteins,chromosome localization and phylogenetic evolutionary relationships were analyzed.Artificial intelligence software AlphaFold 3 was used to predict the three-dimensional structure of Toll receptors and compare it with the known structures and functions of Toll receptors from other species to predict their potential functions and interspecific functional differentiation.Transcriptome data were used to quantitatively analyze the expressions of Toll receptor genes in different tissues and at different developmental stages.【Result】A total of 6,7,and 6 Toll receptor genes were identified in the genomes of N.lugens,S.furcifera,and L.striatellus,respectively,all of which are distributed on chromosomes 1,4,and 7,with a clear distribution pattern.The Toll gene family in the three species of rice planthoppers is distributed with one gene on chromosomes 1 and 4,and the rest on chromosome 7.The coding sequence lengths of the Toll receptor genes in the three species of rice planthoppers range from 2676 to 4158 bp,with the number of exons ranging from 1 to 7,and the encoded protein sequence lengths range from 891 to 1385 aa,with molecular weights ranging from 103.31 to 158.25 kDa and theoretical isoelectric points ranging from

关 键 词：稻飞虱 TOLL受体 免疫发育 基因家族鉴定 

分 类 号：S435.112.3[农业科学—农业昆虫与害虫防治]