基于垂体全长转录组测序分析鸡睾丸性状相关基因  

Full-Length Transcriptomic Analysis of Chicken Pituitary Reveals Candidate Genes for Testicular Trait

作　　者：马荆鄂 熊信威 周敏[1] 吴斯琪 韩甜 饶友生[1] 王樟凤[1] 许继国 MA JingE;XIONG XinWei;ZHOU Min;WU SiQi;HAN Tian;RAO YouSheng;WANG ZhangFeng;XU JiGuo(Institution of Biological Technology,Nanchang Normal University/JiangXi Province Key Lab of Genetic Improvement of Indigenous Chicken Breeds,Nanchang 330032)

机构地区：[1]南昌师范学院生物技术研究院/家禽遗传改良江西省重点实验室,南昌330032

出　　处：《中国农业科学》2024年第20期4130-4144,I0002-I0004,共18页Scientia Agricultura Sinica

基　　金：国家自然科学基金(32160784);江西省教育厅科技计划(GJJ2202002);江西省重点研发计划(20212BBF63028,20212BBF61011)。

摘　　要：【背景】睾丸是雄性动物重要生殖器官,具有产生精子和分泌雄激素的功能,睾丸质量与繁殖性能有直接联系。睾丸性状受下丘脑-垂体-性腺轴多基因共同调控,其分子机理尚不清楚。宁都黄鸡睾丸性状变异系数大,繁殖性能一致性差。【目的】通过对不同睾丸性状个体垂体组织进行全长转录组测序,挖掘候选基因以及关键信号通路,探究影响睾丸性状的遗传基础,以期为发展地方鸡种睾丸性状的选种选育提供依据。【方法】以70只22周龄宁都黄公鸡为研究对象,分为大睾丸组和小睾丸组,从两组中分别选取3个个体,构建垂体组织的全长转录本表达谱,筛选高表达量及两组个体差异表达基因或转录本,对差异基因或转录本进行功能富集分析。随机选取6个基因,进行基因表达水平验证。参考已知垂体细胞的标记基因,筛选与8种细胞标记基因对应的差异表达基因或转录本,并分析这些差异基因或转录本以及高表达量基因或转录本与睾丸性状相关性。【结果】在6个宁都黄鸡垂体组织中,检测到21834个基因,29355个全长转录本。其中,332个基因及229个转录本在两组个体中差异表达。KEGG通路分析结果显示,这些差异基因或转录本主要富集于Apelin信号、甘油磷脂代谢等通路。一共发现5个促性腺细胞标记基因或其对应转录本与部分睾丸性状显著相关,包括NFASC(neurofascin)及其对应转录本XM_015298904.2、TESC(tescalcin)及其对应转录本XM_025155510.1、FSHB(folliclestimulatinghormonebeta)转录本ONT.20974.4、RLN3(relaxin 3)、NDUFB2(NADH:ubiquinone oxidoreductase subunit B2)转录本ONT.1176.4。1个催乳素细胞标记基因EGR1(early growth response 1),2个滤泡星状细胞标记基因TMSB4X(thymosin beta 4,X-linked)、C1H12ORF57(chromosome 1 open reading frame),1个促甲状腺细胞标记基因CHGB(chromogranin B)均与部分睾丸性状显著相关。2个高表达量基因TMSB4X、HS【Background】Testis is an important reproductive organ of male animals,which has the function of producing sperm and secreting androgen.The quality of testis is directly related to reproductive performance.Testicular traits are regulated by multiple genes in the hypothalamic-pituitary-gonadal axis,but the molecular mechanism is still unclear.The variation coefficient of testicular traits was large in Ningdu yellow chicken,with poor reproductive performance consistency.【Objective】In order to explore candidate genes and key signaling pathways,full-length transcriptome sequencing was performed on pituitary tissues of six individuals with different testicular traits.The selection and breeding of testis traits was expected to develop local chicken breeding with these founding genetic basis.【Method】A total of seventy 22-week-old Ningdu yellow roosters were studied.Six individuals were randomly selected,including three individuals with large testicular and others with small testicular.Full-length transcript expression profile was constructed on pituitary tissue for each individual.Then the genes or transcripts were selected with high expression or different expression between two groups of individuals.Gene function enrichment analysis was also performed for these differentially expressed genes or transcripts.Six genes were randomly selected to verify the gene expression level.Referred to known marker genes in pituitary cells,screening the differentially expressed genes or transcripts were carried out corresponding to the marker genes in the eight kinds of cells.The correlation was analyzed between testicular traits and these genes or transcripts and also those with high expressional level.【Result】In six pituitary tissues,21834 genes and 29355 full-length transcripts were detected.Among them,332 genes and 229 transcripts were differentially expressed between the two groups.KEGG pathway analysis showed that differentially expressed genes or transcripts were mainly concentrated in apelin signaling pathway,an

关 键 词：转录组测序 宁都黄鸡 睾丸性状 候选基因 

分 类 号：S831.2[农业科学—畜牧学]