解郁安神颗粒一测多评多组分定量检测法建立及综合质量评价模型构建  

Establishment of QAMS Multi-component Quantitative Detection Method for Jieyu Anshen Granules and Construction of Comprehensive Quality Evaluation Model

作　　者：万刘静[1] 张利 WAN Liujing;ZHANG Li(School of Health,Chongqing Industry&Trade Polytechnic,Fuling 408000,China;School of Chemical Engineering,Sichuan University of Science&Engineering,Zigong 643002,China)

机构地区：[1]重庆工贸职业技术学院健康学院,涪陵408000 [2]四川轻化工大学化学工程学院,自贡643002

出　　处：《医药导报》2024年第11期1827-1832,共6页Herald of Medicine

基　　金：四川省科技计划重点研发项目(2019YFS0095)。

摘　　要：目的建立一测多评(QAMS)法同时测定解郁安神颗粒中13种成分含量,并构建其综合质量评价模型。方法以乙腈-0.1%磷酸为流动相,同时测定解郁安神颗粒中王百合苷C、王百合苷E、王百合苷B、羟异栀子苷、栀子苷、斯皮诺素、酸枣仁皂苷A、酸枣仁皂苷B、柴胡皂苷a、柴胡皂苷d、双去甲氧基姜黄素、去甲氧基姜黄素和姜黄素的含量,采用正交偏最小二乘法-判别分析(OPLS-DA)和熵权逼近理想解排序(EW-TOPSIS)法对其整体质量进行综合评价。结果高效液相色谱(HPLC)方法学验证均符合《中华人民共和国药典》要求。OPLS-DA显示栀子苷、柴胡皂苷d、王百合苷B、姜黄素、斯皮诺素和柴胡皂苷a为解郁安神颗粒质量差异的主要因子。EW-TOPSIS显示14批解郁安神颗粒最优解欧氏贴近度0.2849~0.6407。结论所建立的方法可用于解郁安神颗粒的质量控制和整体评价。Objective To establish a method for simultaneous determination of 13 components in Jieyu Anshen granules by quantitative analysis of multi components by single marker(QAMS),and to construct its comprehensive quality evaluation model.Methods The mobile phase was acetonitrile-0.1%phosphoric acid,the contents of regaloside C,regaloside E,regaloside B,gardenoside,geniposide,spinosin,jujuboside A,jujuboside B,saikosaponin a,saikosaponin d,bisdemethoxycurcumin,desmethoxycurcumin and curcumin in Jieyu Anshen granules were determined.Orthogonal partial least squares-discriminant analysis(OPLS-DA)and entropy weight-technique for order preference by similarity to an ideal solution(EW-TOPSIS)methods were used for comprehensive quality evaluation.Results The methodological verification met the requirements of Chinese Pharmacopoeia.The orthogonal partial least squares-discriminant analysis(OPLS-DA)showed that geniposide,yayoisaponin d,regaloside B,curcumin,spinosin and saikosaponin a were the main factors for the quality difference of Jieyu Anshen granules.The EW-TOPSIS method showed that the average values of C i of 14 batches of samples were 0.2849-0.6407.Conclusion The established method can be used for the quality control and overall evaluation of Jieyu Anshen granules.

关 键 词：解郁安神颗粒 一测多评法 正交偏最小二乘法-判别分析 熵权优劣解距离法 质量评价 

分 类 号：R286[医药卫生—中药学] R927.1[医药卫生—中医学]