G-quadruplex is involved in the regulation of BmSGF1 expression in the Silkworm, Bombyx mori  

作　　者：Yanfei Chen Jin Li Kangkang Niu Yuling Peng Qisheng Song Qili Feng 

机构地区：[1]School of Biology and Agriculture,Shaoguan University,Shaoguan,China [2]Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,Institute of Insect Science and Technology,School of Life Sciences,South China Normal University,Guangzhou,China [3]Guangzhou Key Laboratory of Insect Development Regulation and Application Research,Institute of Insect Science [4]Technology,School of Life Sciences,South China Normal University,Guangzhou,China and 4Division of Plant Science&Technology,UniversityofMissouri,Columbia,Missouri,USA

出　　处：《Insect Science》2024年第5期1440-1452,共13页昆虫科学（英文版）

基　　金：This research was supported by the National Natural Science Foundation of China(31930102,32250710148,32000337,32100383);Shaoguan University high-level talent research start-up funding project(432/9900064607);Research Projects of Shaoguan University(SY2023KJ03).

摘　　要：Advanced DNA structures,such as the G-quadruplex(G4)and the i-motif,are widely but not randomly present in the genomes of many organisms.A G4 structure was identified in the promoter of the silk gland factor-1 gene(SGFI),which is the main regulatory gene for silk production in Bombyx mori.In this study,a BmSGF1 G4^(-/-)ho-mozygous mutant was generated with the G4 sequence knocked out.The promoter activity of BmSGF1 was lowered in the BmSGF1 G4^(-/-)mutant.Pyridostatin(PDS)stabilized the G4 structure and increased the promoter activity of BmSGF1,whereas anti-sense oligonu-cleotide(ASO)complementary to the G4 sequence suppressed the promoter activity of BmSGF1.Compared with wild-type larvae,the deletion of the BmSGF1 G4 structure de-creased both the expression of BmSGF1 and the fibroin heavy chain gene BmFib-H in the posterior silk gland and the weight of the cocoons.Overall,these results suggest that the promoter G4 structure of BmSGFI participates in the transcription regulation of the BmSGFl gene in the silkworm.

关 键 词：BmSGF1 G4 Bombyx mori CRISPR/Cas9 silk production 

分 类 号：S881.2[农业科学—特种经济动物饲养]