Insulin receptor participates in the peripheral olfactory processes of honey bees (Apis cerana cerana)  

作　　者：Huiting Zhao Longlong Chen Miaomiao Liu Shuguo Zhao Weihua Ma Yusuo Jiang 

机构地区：[1]Colege of Life Science,Shanxi Agricultural University,Taigu,Shanxi,China [2]College of Horticulture,Shanxi Agricultural University,Taiyuan,China [3]College of Animal Science,Shanxi Agricultural University,Taigu,Shanxi,China

出　　处：《Insect Science》2024年第5期1477-1488,共12页昆虫科学（英文版）

基　　金：This research was funded by the earmarked fund for China Agriculture Research System(grant number CARS-44-KXJ22);the Natural Science Foundation of Shanxi Province,China(grant numbers:201901D211356 and 20210302123369).

摘　　要：Insulin receptors(InR)are an integral component of the insulin/insulin-like growth factor signaling pathway,which plays a vital role in insect development,lifespan,reproduction,and olfactory sensitivity.However,whether InR participate in the periph-eral olfactory system of insects remains unclear.Recently,we found that 2-heptanone(2-HT)affects AcerlnR expression,the gene for an InR protein,in Apis cerana cerana.We then examined the spatiotemporal expression profile of the gene in A.cerana cerana.The mRNA of AcerlnR was primarily expressed in the antennae,wings,and legs of forager bees,which are probable chemosensory tissues.The results of fluorescence competitive binding assays,combined with site-directed mutagenesis,demonstrated that AcerOBP6 and AcerOBP14 exhibit strong binding affinities to 2-HT.Furthermore,after foragers were fed with double-stranded AcerlnR,the expression levels of AcerOBP6 and AcerOBP14 decreased significantly,as did the electroantennogram responsiveness to 2-HT and some other odorants.In conclusion,our findings provide a foundation for understanding the in-volvement of AcerlnR in the odor perception of A.cerana cerana.Moreover,they offer novel insights into the olfactory recognition mechanism in insects.

关 键 词：ANTENNA Apis cerana cerana insulin receptor odorant-binding protein ol-faction 

分 类 号：S891[农业科学—特种经济动物饲养]