High-throughput molecular gut content analysis of aphids identifies plants relevant for potato virus Y epidemioiogy  

作　　者：William Jacob Pitt William Rodney Cooper Derek Pouchnik Heather Headrick Punya Nachappa 

机构地区：[1]Department of Agricultural Biology,Colorado State University,Fort Collins,Colorado,USA [2]Graduate Degree Program in Ecology,Colorado State University,Fort Collins,Colorado,USA [3]USDA-ARS Temperate Tree Fruit and Vegetable Research Unit,Wapato,Washington,USA [4]Laboratory for Biotechnology and BioanalysisWashington State University,Pullman,Washington,USA [5]Tree Fruit Research and Extension Center,Washington State University,Wenatchee,Washington,USA

出　　处：《Insect Science》2024年第5期1489-1502,共14页昆虫科学（英文版）

摘　　要：Aphids are phloem-feeding insects that reduce crop productivity due to feeding and transmission of plant viruses.When aphids disperse across the landscape to colonize new host plants,they will often probe on a wide variety of nonhost plants before settling on a host suitable for feeding and reproduction.There is limited understanding of the diversity of plants that aphids probe on within a landscape,and characterizing this diversity can help us better understand host use patterns of aphids.Here,we used gut content analysis(GCA)to identify plant genera that were probed by aphid vectors of potato virus Y(PVY).Aphids were trapped weekly near potato fields during the growing seasons of 2020 and 2021 in San Luis Valley in Colorado.High-throughput sequencing of plant barcoding genes,trnF and ITS2,from 200 individual alate(i.e.,winged)aphids representing nine vector species of PVY was performed using the PacBio sequencing platform,and sequences were identi-fied to genus using NCBI BLASTn.We found that 34.7%of aphids probed upon presumed PVY host plants and that two of the most frequently detected plant genera,Solanum and Brassica,represent important crops and weeds within the study region.We found that 75%of aphids frequently probed upon PVY nonhosts including many species that are outside of their reported host ranges.Additionally,19%of aphids probed upon more than one plant species.This study provides the first evidence from high-throughput molecular GCA of aphids and reveals host use patterns that are relevant for PVY epidemiology.

关 键 词：APHIDS EPIDEMIOLOGY gut content analysis insect ecology plant-insect in-teractions plant disease 

分 类 号：Q96[生物学—昆虫学]