Functional types of long trichoid sensilla responding to sex pheromone components in Plutella xylostella  

作　　者：Xiao-Fei Li Xing-Tao Qie Bao-Tong Mo Cai-Feng Wang Zeng-Hua Xing Jin-Yu Zhao Chen-Zhu Wang Chi Hao Li Ma Xi-Zhong Yan 

机构地区：[1]College of Plant Protection,Shanxi Agricultural University,Taigu,Shanxi Province,China [2]State Key Laboratory of Integrated Management of Pest Insects and Rodents,Institute of Zoology,Chinese Academy of Sciences,Bejing,China [3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,China

出　　处：《Insect Science》2024年第5期1503-1518,共16页昆虫科学（英文版）

基　　金：This study was supported by the National Natural Science Foundation of China(Grant No.32130090,32300403);the Distinguished and ExcellentYoung Scholar Cultivation Project of Shanxi Agricultural University(2023YQPYGC05);the Leading Industrial and Engineering Research Project of Shanxi Agricultural University(Grant No.CYYL23-25);the Cultivation and Innovation Project of Plant Protection College of Shanxi Agricultural University(Grant No.ZBXY23B-13).

摘　　要：Sex pheromones,which consist of multiple components in specific ratios pro-mote intraspecific sexual communications of insects.Plutella xylostella(L.)is a world-wide pest of cruciferous vegetables,the mating behavior of which is highly dependent on its olfactory system.Long trichoid sensilla on male antennae are the main olfactory sen-silla that can sense sex pheromones.However,the underlying mechanisms remain unclear.In this study,3 sex pheromone components from sex pheromone gland secretions of P xy-lostella female adults were identified as Z11-16:Ald,Z11-16:Ac,and Z11-16:0H in a ratio of 9.4:100:17 using gas chromatography-mass spectrometry and gas chromatography with electroantennographic detection.Electrophysiological responses of 581 and 385 long trichoid sensilla of male adults and female adults,respectively,to the 3 components were measured by single sensillum recording.Hierarchical clustering analysis showed that the long trichoid sensilla were of 6 different types.In the male antennae,52.32%,5.51%,and 1.89%of the sensilla responded to Z11-16:Ald,Z11-16:Ac,and Z11-16:0H,which are named as A type,B type,and C type sensilla,respectively;2.93%named as D type sen-silla responded to both Z11-16:Ald and Z11-16:Ac,and 0.34%named as E type sensilla were sensitive to both Z11-16:Ald and Z11-16:OH.In the female antennae,only 7.53%of long trichoid sensilla responded to the sex pheromone components,A type sensilla were 3.64%,B type and C type sensilla were both 0.52%,D type sensilla were 1.30%,and 1.56%of the sensilla responded to all 3 components,which were named as F type sen-silla.The responding long trichoid sensilla were located from the base to the terminal of the male antennae and from the base to the middle of the female antennae.The pheromone mixture(Z11-16:Ald:Z11-16:Ac:Z11-16:0H=9.4:100:17)had a weakly repellent effect on female adults of P xylostella.Our results lay the foundation for further studies on sex pheromone communications in P.xylostella.

关 键 词：electrophysiological responses long trichoid sensilla Plutella xylostella sex pheromone single sensillum recording 

分 类 号：Q96[生物学—昆虫学]