超高效液相色谱-质谱联用法测定人全血中羟氯喹的浓度  

Determination of Hydroxychloroquine in Human Whole Blood by UPLC-MS/MS

作　　者：卢志品[1] 伊金玲 曹伟[1] LU Zhi-ping;YI Jin-ling;CAO Wei(First Affiliated Hospital of Xiamen University,Xiamen 361003,China;Accu BE Pharm Technology Limited Liability Company,Xiamen 361026,China)

机构地区：[1]厦门大学附属第一医院,福建厦门361003 [2]莱必宜科技(厦门)有限责任公司,福建厦门361026

出　　处：《海峡药学》2024年第10期59-62,共4页Strait Pharmaceutical Journal

摘　　要：目的建立超高效液相色谱串联质谱测定人全血中羟氯喹浓度的方法。方法全血样品经乙腈蛋白沉淀后,上清液采用超高效液相色谱串联质谱仪测定。色谱柱为ACQUITY UPLCHSS T3色谱柱(2.1×50 mm,1.8μm),流动相A为含0.3%甲酸、3 mmol·L^(-1)甲酸铵的水溶液,流动相B为含0.3%甲酸、3 mmol·L^(-1)甲酸铵的乙腈∶甲醇∶水(V∶V∶V,95∶95∶10)混合溶液,进行梯度洗脱,总流速为0.30 mL·min^(-1),使用电喷雾正离子模式,多反应离子监测。结果在1~500 ng·mL^(-1)范围内,羟氯喹具有良好的线性关系,批内、批间RSD均小于10%。结论本试验所建立的分析方法准确、灵敏、方便,可用于羟氯喹的人体药动学研究。OBJECTIVE To establish the determination method of hydroxychloroquine in human whole blood with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).METHODS Whole blood samples were precipitated by acetonitrile,then the supernatant was determinated by UPLC-MS/MS.Column ACQUITY UPLCHSS T3(2.1×50 mm,1.8μm)was used with mobile phase A of water(containing 0.3%formic and 3 mmol·L^(-1) ammonium formate)and mobile phase B of acetonitrile∶methanol∶water(V∶V∶V,95∶95∶10)(containing 0.3%formic and 3 mmol·L^(-1) ammonium formate),with the gradient elution at a flow rate of 0.30 mL·min^(-1).Electrospray positive ion model and multiple reaction monitoring were used.RESULTS There was a good linerrelationship in the calibration curve of 1-500 ng·mL^(-1) for hydroxychloroquine,and the intra-day and inter-day relative standard deviations were less than 10%.CONCLUSION The UPLC-MS/MS method was accuracy,sensitive and simple,and can be applied to a pharmacokinetics of hydroxychloroquine in human.

关 键 词：超高效液相色谱-质谱 人全血 羟氯喹 

分 类 号：R969[医药卫生—药理学]