阴极穿透式反应器耦合电活化高锰酸钾体系同步去除多重耐药细菌及抗性基因  

Simultaneous removal of ARB and ARGs by cathode flow-through reactor coupled with electro-activated permanganate process

作　　者：张晓晖 孙威 卞静 谢沐杉 钟艺华 赵纯[1] ZHANG Xiaohui;SUN Wei;BIAN Jing;XIE Mushan;ZHONG Yihua;ZHAO Chun(Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment,Ministry of Education,Chongqing University,Chongqing 400045,China;School of Life Sciences,Southwest University,Chongqing 400715,China;Department of Gastroenterology,Chongqing University Cancer Hospital,School of Medicine,Chongqing University,Chongqing 400030,China)

机构地区：[1]重庆大学,三峡库区生态环境教育部重点实验室,重庆400045 [2]西南大学生命科学学院,重庆400715 [3]重庆大学医学院附属重庆大学肿瘤医院消化内科,重庆400030

出　　处：《环境工程学报》2024年第8期2189-2201,共13页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金面上资助项目(22076015);重庆市医学科研项目(2021MSXM083)。

摘　　要：抗生素耐药性的问题已在全球范围内引起广泛关注,特别是在水环境中的传播。为了应对这一挑战,本研究建立了阴极穿透式反应器,探究了电活化高锰酸钾(PM)对抗生素去除、多重耐药大肠杆菌灭活以及抗性基因削减的效果和作用机制。探究了PM投加量、电流密度、初始p H及实际水体和水体中常见物质(HA、Cl^(-)、HCO_(3)^(-)、HPO_(4)^(2-))对多重耐药大肠杆菌灭活的影响。结果表明,当PM浓度仅为7μmol·L^(–1)、电流密度为12.55 m A·cm^(–2)、初始p H为5时,电阴极-高锰酸钾体系具有明显的协同消毒效果,反应15 min时大肠杆菌去除率可达4.92 log。同时对该细菌携带的抗性基因具有显著的去除效果,对5种抗性基因实现高达3.89 log的对数去除率(99.99%),并且可同时高效去除水中抗生素,可达96.4%的盐酸四环素去除率。利用扫描电镜可观察到处理后的细菌受到严重损伤,细胞膜完整性受到破坏。通过细胞表征进一步发现细胞膜上的脂质被氧化,同时胞内物质逐渐释放并被降解,大肠杆菌完全失活。通过活性锰物种检测和掩蔽实验分析该体系中起主要消毒作用的活性物种为Mn(Ⅲ)_(aq)。该体系在实际水体中表现出优异的多重耐药大肠杆菌去除能力。Cl^(–)对多重耐药大肠杆菌的灭活几乎没有影响,HA、HCO_(3)^(-)、HPO_(4)^(2-)对其灭活存在抑制作用。以上研究结果可对遏制抗生素耐药性在水环境中的传播提供参考。The issue of antibiotic resistance has received worldwide attention,especially its spread in water environment.To address this challenge,a cathode flow-through reactor was established in this study,then the effects and mechanisms of electro-activated permanganate on antibiotic removal,inactivation of antibiotic resistant Escherichia coli(AR E.coli),and reduction of resistance genes(ARGs)were investigated.The effects of permanganate dosage,current density,initial pH value,actual water bodies and water matrices,including humic acid(HA),chloride ion(Cl^(–)),bicarbonate(HCO_(3)^(–)),hydrogen phosphate(HPO_(4)^(2–)),on the inactivation of AR E.coli were investigated.The results showed electric cathode-permanganate process had significant synergistic disinfection effects at the permanganate concentration of only 7μmol·L^(–1),the current density of 12.55 mA·cm^(–2),and the initial pH 5,achieving 4.92 log removal rate after 15 minutes.Meanwhile,the process exhibited a significant removal effect on the ARGs carried by the bacteria,resulting in a 3.89 log reduction(99.99%)in five ARGs.In addition,this process can also efficiently remove antibiotics,and could remove 96.4%tetracycline.Scanning electron microscopy images revealed that the treated bacteria were severely damaged and their cell membrane integrity was disrupted.Through further confirmation,it was found that lipid on the cell membrane were oxidized,while intracellular substances were gradually released and degraded,resulting in complete inactivation of AR E.coli.Detection of reactive manganese species and masking experiments identified Mn(III)aq as the primary disinfecting active species.In actual water,the process showed an excellent removal ability of AR E.coli.The presence of Cl^(–)had no effect on the inactivation of AR E.coli,while HA,HCO_(3)^(–)and HPO_(4)^(2–)all inhibited the inactivation process.The above result provides a reference for inhibit the spread of antibiotic resistance in aquatic environments.

关 键 词：耐药细菌 消毒 抗性基因 高锰酸钾 电化学 

分 类 号：X703[环境科学与工程—环境工程]