压力场作用下填埋场生活垃圾组分对其中细菌群落的影响  

The influence of landfill domestic waste components on bacterial communities under pressure field

作　　者：宁成奇 成兆文 王辉 黄秋杰 杨常富 王罗春 王湘徽 孙梦圆 张珊 楼紫阳[3] NING Chengqi;CHENG Zhaowen;WANG Hui;HUANG Qiujie;YANG Changfu;WANG Luochun;WANG Xianghui;SUN Mengyuan;ZHANG Shan;LOU Ziyang(College of Environmental and Chemical Engineering,Shanghai Electric Power University,Shanghai 201306,China;School of Resources Environment and Safety Engineering,University of South China,Hengyang 200241,China;School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200241,China;SUS Environment,Shanghai 201703,China)

机构地区：[1]上海电力大学环境与化学工程学院,上海201306 [2]南华大学资源环境与安全工程学院,衡阳421001 [3]上海交通大学环境科学与工程学院,上海200241 [4]上海康恒环境修复有限公司,上海201703

出　　处：《环境工程学报》2024年第8期2300-2310,共11页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(42077111);国家重点专项资助项目(2018YFC1800600);“科技兴蒙”上海交通大学行动计划专项项目(2021PT0045-02-01);高端外国专家项目(G2022037007L)。

摘　　要：填埋场作为典型的生物反应器,其微生物群落受到多种作用的影响。探究填埋场中微生物群落结构与压力场、生活垃圾有机组分之间的关系,将为生物强化技术的开发提供理论依据。以浙江某实际填埋场为例,运用网络分析、中性群落模型以及随机森林算法,探究了不同上覆压力和有机组分对垃圾中微生物特征的影响。结果显示:垃圾有机质含量和生物可降解度(BDM)随上覆压力增加而减小。Firmicutes、Proteobacteria、Bacteroidetes和Actinobacteriota等是群落中的优势门类,细菌群落与上覆压力(R=0.51,p<0.01)和BDM(R=0.31,p<0.05)具有相关性。群落内合作最为紧密、网络结构最稳定的是上覆压力低于30 kPa的填埋层,随机性过程在这一压力区间内对群落组装的贡献度最小,这可能与该区域的氧气条件和有机组分特征有关。Enterococcus被识别为填埋场有机组分降解过程中潜在的生物标志物。研究结果有助于深入理解填埋场稳定化过程。Landfills is a typical bioreactors,and its microbial communities are influenced by various factors.Exploring the relationship between microbial community structure in landfills and pressure field,organic fractions of municipal solid waste,will provide a theoretical basis for the development of bioremediation technologies.Taking a landfill site in Zhejiang as an example,this study explored the impact of different overburden pressures and organic components on the characteristics of microbial communities in waste using network analysis,neutral community models,and random forest algorithms.The results showed that the content of organic matter and the biodegradability(BDM)of the waste decreased with increasing overburden pressure.Firmicutes,Proteobacteria,Bacteroidetes,and Actinobacteriota were the dominant phyla in the community.There was a correlation between bacterial communities and overlying pressure(R=0.51,p<0.01)and BDM(R=0.31,p<0.05).The most closely cooperating and stable network structures within the community were found in landfill layers with an overburden pressure below 30 kPa.Random processes contributed least to community assembly,possibly related to the oxygen conditions and organic component characteristics of the area.Enterococcus was identified as a potential biomarker in the degradation process of organic components in landfills.These findings contribute to a deeper understanding of the stabilization process in landfills.

关 键 词：填埋场 垃圾有机组分 压力场 细菌群落结构 群落组装 

分 类 号：X705[环境科学与工程—环境工程]