艾灸通过调节滑膜G蛋白偶联受体43和辅助性T细胞17/调节性T细胞平衡改善佐剂性关节炎大鼠滑膜炎性反应  

Effects of moxibustion in improving synovitis in adjuvant arthritis rats by regulating synovial GPR43 and peripheral blood Th17/Treg balance

作　　者：霍晨露 刘安婷 李双 王淼 朱艳[2] HUO Chen-lu;LIU An-ting;LI Shuang;WANG Miao;ZHU Yan(Graduate School of Anhui University of Chinese Medicine,Hefei 230031,China;Department of Geriatrics I,The Second Affiliated Hospital of Anhui University of Chinese Medicine,Hefei 230061)

机构地区：[1]安徽中医药大学研究生院,合肥230031 [2]安徽中医药大学第二附属医院老年一科,合肥230061

出　　处：《针刺研究》2024年第10期1056-1062,共7页Acupuncture Research

基　　金：国家自然科学基金项目(No.81403484);安徽省高校自然科学基金项目(No.KJ2019A0448);国家级项目培育基金项目计划(No.2019py01);安徽省临床医学研究中心项目(No.安徽省科技厅皖社科[2020]41号);安徽省中医药传承创新科研项目(No.2020ccyb13)。

摘　　要：目的:观察艾灸对佐剂性关节炎(AA)大鼠G蛋白偶联受体43(GPR43)和辅助性T细胞17(Th17)/调节性T细胞(Treg)平衡的影响,探讨艾灸治疗类风湿关节炎(RA)的部分机制。方法:Wistar大鼠随机分为正常组、模型组和艾灸组,每组8只。采用风、寒、湿环境因素加弗氏完全佐剂注射法复制AA大鼠模型。艾灸组艾灸“肾俞”“足三里”,20 min/次,1次/d,持续21 d。观察各组大鼠关节肿胀度(JSD)、关节炎指数(AI)变化;采用透射电镜和HE染色观察各组大鼠踝关节滑膜细胞结构的改变;Western blot法检测大鼠踝关节滑膜组织中GPR43蛋白表达水平;流式细胞术检测外周血中Treg和Th17的百分比;ELISA法检测血清中白细胞介素-10(IL-10)和生长转化因子-β1(TGF-β1)含量;对滑膜组织GPR43与外周血Treg的表达进行相关性分析。结果:与正常组相比,模型组JSD和AI均升高(P<0.01),滑膜病理和超微结构损伤明显,滑膜组织中GPR43蛋白表达降低(P<0.01),外周血中Treg百分比降低(P<0.01),Th17百分比升高(P<0.01),血清IL-10、TGF-β1含量降低(P<0.01)。与模型组相比,艾灸组的JSD和AI均降低(P<0.01),滑膜病理和超微结构损伤程度降低,滑膜组织中GPR43蛋白表达升高(P<0.05),外周血中Treg百分比升高(P<0.01),Th17百分比降低(P<0.01),血清IL-10、TGF-β1含量升高(P<0.01)。滑膜组织GPR43蛋白相对表达量与外周血Treg百分比呈正相关(r=0.967,P<0.01)。结论:艾灸可显著改善AA大鼠滑膜炎性反应,其作用机制可能与调控GPR43从而恢复Th17/Treg平衡有关。Objective To observe the effects of moxibustion on G protein-coupled receptor 43(GPR43)and helper T cell 17(Th17)/regulatory T cell(Treg)balance in rats with adjuvant arthritis(AA),so as to investigate the partial mechanism of moxibustion therapy on rheumatoid arthritis(RA).Methods A total of 24 Wistar rats were randomly divided into a normal group,a model group and a moxibustion group,with 8 rats in each group.The AA rat model was replicated using wind,cold and moisture environmental factors+Freund’s complete adjuvant(CFA)injection method.In the moxibustion group,moxibustion was performed on bilateral“Zusanli”(ST36)and“Shenshu”(BL23)for 20 min/time,once daily,for 21 d.The changes of joint swelling degree(JSD)and arthritis index(AI)were observed in each group of rats.Transmission electron microscopy and HE staining were used to examine changes in the cellular structure of the ankle joint synovial tissue in each group.Western blot analysis was employed to detect the expression levels of GPR43 in the synovial tissue of the ankle joints.Flow cytometry was used to measure the percentages of Treg and Th17 in peripheral blood.ELISA was used to determine the contents of interleukin-10(IL-10)and transforming growth factor-β1(TGF-β1)in serum.Results Compared with the normal group,the JSD and AI in the model group increased before and after treatment(P<0.01),with significant synovial pathological and ultrastruct ural injury observed.Additionally,compared with the normal group,the expression of GPR43 in the synovial tissue decreased(P<0.01),the Treg percentage in peripheral blood decreased(P<0.01)and the Th17 percentage increased(P<0.01),serum IL-10 and TGF-β1 contents decreased in the model group(P<0.01).Compared with the model group,the moxibustion group exhibited a significant reduction in JSD and AI after treatment(P<0.01),the degree of pathological and ultrastruct ural damage in the synovium decreased,the expression of GPR43 in the synovial tissue increased(P<0.05),the Treg percentage in peripheral blood i

关 键 词：艾灸 G蛋白偶联受体43 辅助性T细胞17/调节性T细胞平衡 佐剂性关节炎 

分 类 号：R245[医药卫生—针灸推拿学]