大黄灵仙方对胆管炎症大鼠IL-6、EGR-1相关蛋白表达的影响  

Effect of Rhubarb Lingxian on expression of IL-6 and EGR-1 related proteins in rats with bile duct inflammation

作　　者：王兵[1] 许彦 黄欣[1] 黄汉基 曾鸿孟 WANG Bing;XU Yan;HUANG Xin;HUANG Han-ji;ZENG Hong-meng(First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi,530023,China;Loudi Traditional Chinese Medicine Hospital,Loudi,Hunan,417000,China)

机构地区：[1]广西中医药大学第一附属医院,广西南宁530023 [2]娄底市中医医院,湖南娄底417000

出　　处：《时珍国医国药》2024年第10期2311-2315,共5页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金项目(81804099,82360889);广西自然科学基金(2020GXNSFBA297093、2021GXNSFBA220059);“岐黄工程”高层次人才团队培育项目肝胆相关疾病的中西医防治研究团队(202411)。

摘　　要：目的通过在没有胆囊的大鼠上构建急性肝胆管炎症模型,探究大黄灵仙方干预肝内胆管结石形成的作用机制。方法取40只雄性SD大鼠随机分为空白正常组、脂多糖模型组、熊去氧胆酸胶囊对照组、大黄灵仙方实验组,每组各10只。正常组予常规饲料饲养;模型组、对照组和实验组在胆总管一次性注射1.25mg/kg脂多糖,同时对照组予熊去氧胆酸灌胃干预,实验组予大黄灵仙方灌胃干预,共7d。取血液进行ELISA检测,采取肝脏组织进行Masson染色、WB检测IL-6和EGR-1蛋白表达的情况。结果与其他三组相比,模型组胆管细胞纤维化程度明显;各组大鼠血液及肝脏组织IL-6、EGR-1蛋白表达总体差异有统计学意义(P<0.01);与正常组相比较,模型组和对照组血液及肝脏组织IL-6、EGR-1蛋白表达差异均明显增高(P<0.01);与正常组相比较,实验组血液及肝脏IL-6、EGR-1蛋白表达无明显差异(P>0.05)。结论大黄灵仙颗粒可能通过抑制胆管炎症大鼠模型IL-6、EGR-1等炎症因子的释放,减轻肝损伤,使之趋于正常水平,从而达到阻断结石的形成。Objective To explore the mechanism of Dahuang Lingxian Recipe on the formation of intrahepatic bile duct stones on the basis of constructing the model of hepatobiliary inflammation in SD rats.Methods Forty male SD rats were randomly divided into blank group,lipopolysaccharide model group,ursodeoxycholic acid capsule control group and rhubarb Lingxian formula experimental group,with 10 rats in each group.The normal group was fed with conventional feed;The model group,the control group and the experimental group were injected with 1.25mg/kg lipopolysaccharide in the common bile duct at one time.At the same time,the control group was given ursodeoxycholic acid and the experimental group was given Dahuang Lingxian formula for 7 days.Blood was taken for ELISA,liver tissue was stained with he,and WB was used to detect the expression of IL-6 and Egr-1 protein.Results Compared with the other three groups,the pathological morphology of liver in the model group showed that the degree of inflammatory infiltration was obvious;There was significant difference in the expression of IL-6 and Egr-1 protein in blood and liver of rats in each group(P<0.01);Compared with the normal group,the expression of IL-6 and Egr-1 protein in blood and liver of the model group and the control group were significantly higher(P<0.01);Compared with the normal group,there was no significant difference in the expression of IL-6 and Egr-1 protein in blood and liver of the experimental group(P>0.05).Conclusion Dahuang Lingxian granule may inhibit the release of inflammatory factors such as IL-6 and Egr-1 in cholangitis rat model,reduce liver injury and make it tend to normal level,so as to block the formation of stones.

关 键 词：炎症模型 大黄灵仙 IL-6 EGR-1 

分 类 号：R285.5[医药卫生—中药学]