麝香含药血清介导MAPK信号通路对骨髓间充质干细胞迁移的影响  

Effects of Musk containing serum on the migration of bone marrow mesenchymal stem cells mediated by MAPK signaling pathway

作　　者：李应福 张亚维[1] 张堃[1] 谢兴文 李宁 施彦龙 潘鑫戊 LI Ying-fu;ZHANG Ya-wei;ZHANG Kun;XIE Xing-wen;LI Ning;SHI Yan-long;PAN Xin-wu(Gansu Provincial Hospital of Traditional Chinese Medicine,Lanzhou 730050 China;Affiliated Hospital of Gansu University of Traditional Chinese Medicine,Lanzhou 730000 China)

机构地区：[1]甘肃省中医院,甘肃兰州730050 [2]甘肃中医药大学附属医院,甘肃兰州730000

出　　处：《时珍国医国药》2024年第10期2330-2336,共7页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金资助项目(81460735,81860864,82160911)。

摘　　要：目的探讨麝香促体外骨髓间充质干细胞(BMSCs)迁移的机制。方法60只SD大鼠,随机分为麝香高、中、低剂量组及空白对照组,制备麝香含药血清及生理盐水血清。15只SD大鼠,分离BMSCs并培养至第3代,通过形态学、表型鉴定、成骨及成脂诱导鉴定BMSCs,鉴定认为成功后,采用麝香含药血清干预体外BMSCs,检测细胞增殖率,加入MAPK信号通路阻断剂,利用Transwell实验检测麝香对BMSCs迁移的影响。结果大鼠BMSCs呈梭形贴壁生长,排列比较规则,细胞形态较一致;表型鉴定:CD44、CD90阳性表达,CD45、CD34阴性表达;细胞可向成骨、成脂分化;与对照组比较,不同浓度麝香组在不同时间段均能提高BMSCs增殖率(P<0.05);与对照组比较,低浓度麝香组在不同时间段增加BMSCs迁移数量(P<0.05),低浓度麝香+PD干预组随着时间延长其迁移数量明显减少,不同时间段组内比较,差异无统计学意义(P>0.05)。结论麝香可促进大鼠外源性BMSCs迁移,其机制可能与活化MAPK/ERK1/2信号通路有关。Objective To investigate the mechanism of Musk promoting the migration of bone marrow mesenchymal stem cells(BMSCs)in vitro.Methods Sixty SD rats were randomly divided into high,medium and low dose group and blank control group.the saline serum and serum of Musk were prepared.Fifteen SD rats,BMSCs were isolated from rats and cultured for the third generation,BMSCs were identified by morphological,phenotypic identification,osteogenesis and adipogenic induction,after the identification of success,BMSCs were treated with Musk serum to detect cell proliferation rate,the effect of Musk on migration of BMSCs was detected by Transwell assay after the addition of MAPK signal pathway blocker.Results The rat BMSCs were spindle shaped adherent growth,arranged regularly and the cell morphology is consistent;phenotype identification:The expression of CD44 and CD90 was negative,and the expression of CD44 and CD34 was negative;the cells can differentiate into osteoblasts and adipocytes;compared with the control group,different concentration of Musk group can improve the proliferation rate of BMSCs at different time paragraph(P<0.05);compared with the control group,low concentration of Musk group increased the number of Musk BMSCs migration in different time(P<0.05),the migration rate of low concentration Musk+PD treatment group was decreased with the prolongation of time,there was no significant difference between groups in different time periods(P>0.05).Conclusion Musk can promote the migration of exogenous BMSCs in rats,the mechanism may be related to the activation of MAPK/ERK1/2 signaling pathways.

关 键 词：麝香 骨髓间充质干细胞 迁移 机制 信号通路 

分 类 号：R285.5[医药卫生—中药学]