基于PI3K/AKT/FoxO1信号通路探讨湿润烧伤膏对糖尿病大鼠创面EMT过程的作用  

MEBO Affects the EMT Process of Diabetic Ulcer Rat’s Wounds through PI3K/AKT/FoxO1 Pathway

作　　者：童丹蕾 马卓飞[2] 姜艳[2] 左远娟 唐乾利[1] TONG Dan-lei;MA Zhuo-fei;JIANG Yan;ZUO Yuan-juan;TANG Qian-li(Graduate School of Guangxi University of Chinese Medicine,Nanning Guangxi 530001,China;Youjiang Medical University for Nationalities/West Guangxi Key Laboratory for Prevention and Treatment High-Incidence Diseases,Baise Guangxi 533000,China)

机构地区：[1]广西中医药大学研究生院,广西南宁530001 [2]右江民族医学院/桂西高发病防治重点实验室,广西百色533000

出　　处：《时珍国医国药》2024年第10期2351-2361,共11页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(81774327);广西医学高层次领军人才培养“139”计划项目资助(桂卫科教发[2018]22号);广西自然科学基金(2020GXNSFBA297052);广西中医药大学研究生教育创新计划(桂中医大研[2022]11号)(YCBXJ2022015)。

摘　　要：目的探究湿润烧伤膏对糖尿病大鼠创面再上皮化过程的促进作用及其机制。方法90只Wistar雄性大鼠用随机数字法分为5组:MEBO组、rb-bFGF组、模型组、对照组、空白组,每组18只。对照组建立急性创面模型;MEBO组、rb-bFGF组、模型组建立糖尿病创面模型。对照组、模型组大鼠创面予以生理盐水外敷治疗;MEBO组、rb-bFGF组分别予以MEBO和rb-bFGF外敷治疗;空白组只做备皮处理。各组创面于第3、7、14天分别随机取6只大鼠,采集创面组织标本并保存。随后进行Masson染色,观察各组胶原纤维生成及再上皮化情况。免疫荧光、蛋白印迹和qRT-PCR方法检测创面组织FoxO1、E-cadherin及Vimentin等因子表达。结果在造模第7、14天,对照组、MEBO组及rb-bFGF组创面愈合率高于模型组(P<0.05),且创面显示胶原纤维生成及再上皮化情况更好。在造模第3天,MEBO组中AKT蛋白及mRNA表达量高于模型组(P<0.05);第7天,MEBO组及rb-bFGF组中PI3K、AKT、FoxO1、Vimentin蛋白及mRNA表达量高于模型组(P<0.05),E-cadherin、Claudin1则低于模型组(P<0.05);第14天,与模型组相比,MEBO组及rb-bFGF组中AKT、FoxO1、Vimentin蛋白及mRNA表达量降低(P<0.05),E-cadherin、Claudin1表达量升高(P<0.05)。结论湿润烧伤膏能促进糖尿病大鼠创面愈合,其可能作用机制是通过激活PI3K/AKT/FoxO1通路,并影响创面组织中E-cadherin、Claudin1、Vimentin表达及EMT转化过程实现的。Objective To explore the promoting effect of MEBO on the process of wound re-epithelization in diabetes rats and its mechanism.Methods 90 male Wistar rats were randomly divided into five groups:MEBO group,rb-bFGF group,model group,control group,blank group and 18 rats in each group.The control group established an acute wound model.The MEBO group,the rb-bFGF group,and the model group established a diabetes wound model.The control group and the model group rats were treated with external application of normal saline.The MEBO group and the rb-bFGF group were treated with external application of MEBO and rb-bFGF respectively.Blank group only performs Hair Removal Treatment.Six rats were randomly selected from each group on the 3rd,7th,and 14th days,and tissue samples of the wounds were collected and stored.Masson staining were performed to observe the morphological changes of wound tissue.Immunofluorescence,Western blotting,and qRT-PCR were used to detect the protein expressions of factors such as FoxO1,E-cadherin,and Vimentin in each group.Results On the 7th/14th day of modeling,the wound healing rate in the control group,MEBO group,and rb-bFGF group was higher than that in the model group(P<0.05),and the results of wound tissue staining were better.On the 3th day of modeling,the expression levels of AKT protein in the MEBO group were higher than those in the model group(P<0.05);On the 7th day,the expression levels of PI3K,AKT,FoxO1and Vimentin protein in the MEBO and rb bFGF groups were higher than those in the model group(P<0.05),while E-cadherin and claudin1 were lower than those in the model group(P<0.05);On the 14th day,compared with the model group,the expression levels of AKT,FoxO1 and Vimentin protein in the MEBO group and rb-bFGF group decreased(P<0.05),while the expression levels of E-Cadherin and Claudin1 increased(P<0.05).Conclusion MEBO significantly promoted the healing of diabetic ulcer wounds,which was possibly achieved by activating PI3K/AKT/FoxO1 signaling pathway to affect the expressions of E-ca

关 键 词：湿润烧伤膏 糖尿病创面 磷脂酰肌醇3-激酶/蛋白激酶B/叉头框蛋白O1 上皮-间质转化 

分 类 号：R285.5[医药卫生—中药学]