黄芪多糖对自身免疫性甲状腺炎NOD.2^(h4)小鼠血清炎症因子及甲状腺组织的影响  

Effect of Astragalus polysaccharide on serum inflammatory factors and thyroid tissue of autoimmune thyroiditis NOD.2^(h4)mice impact

作　　者：邱子洋 文艺[1] 高天舒[2] QIU Zi-yang;WEN Yi;GAO Tian-shu(Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;the Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang 110032,China)

机构地区：[1]辽宁中医药大学,辽宁沈阳110847 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《时珍国医国药》2024年第10期2362-2368,共7页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金面上项目(81874441,82174359);沈阳市科技计划项目公共卫生研发专项(沈科发[2022]14号)。

摘　　要：目的观察黄芪多糖(APS)对自身免疫性甲状腺炎(AIT)NOD.2^(h4)小鼠血清炎症因子、甲状腺抗体和甲状腺组织病理形态的影响。方法选取90只6~8周龄SPF级雌性NOD.H-2^(h4)小鼠,体质量(25±5)g,随机分为空白对照组、模型组、硒制剂组及黄芪多糖低、中、高剂量组,共6组,每组15只。黄芪多糖低、中、高剂量组每日分别予以200,400,800mg·kg^(-1)黄芪多糖灌胃,硒制剂组每日予以0.26mg·kg^(-1)亚硒酸钠片灌胃,空白对照组及模型剂组每日予以等体积蒸馏水灌胃,连续灌胃给药8周。观察小鼠一般状况,酶联免疫试剂盒(Enzyme-Linked ImmunoSorbent Assay,ELISA)检测各组小鼠血清中白细胞介素-6(IL-6)、白细胞介素-10(IL-10)、白细胞介素-12(IL-12)、白细胞介素-17(IL-17)、转化生长因子β1(TGF-β1)、抗甲状腺球蛋白抗体(TgAb)、促甲状腺激素(TSH)水平,HE染色观察各组小鼠甲状腺淋巴细胞浸润情况。结果与空白对照组比较,模型组血清IL-6、IL-12、IL-17、TgAb显著升高,其余各治疗组均有所降低,且差异具有统计学意义(P<0.001)。与空白对照组比较,模型组血清IL-10、TGF-β1水平显著下降,其余各治疗组均有所升高,且差异具有统计学意义(P<0.001)。与空白对照组比较,其余组小鼠血清TSH均有所升高,但各组间差异没有统计学意义,证实非甲亢、甲减期。HE染色可见AIT组小鼠甲状腺淋巴细胞浸润显著,各治疗组甲状腺淋巴细胞浸润情况均有所恢复。结论黄芪多糖可能通过调节甲状腺自身免疫功能,抑制促炎细胞因子释放,减轻甲状腺淋巴细胞炎症浸润程度,起到延缓AIT发生的作用。Objective To observe the effects of Astragalus membranaceus polysaccharide(APS)on serum inflammatory factors,thyroid antibodies and thyroid histopathological morphology in autoimmune thyroiditis(AIT)NOD.2^(h4)mice.Methods A total of 906-8-week-old female NOD.H-2^(h4)mice with a weight of(25±5)g were randomly divided into 6 groups,including blank control group,model group,selenium preparation group and low-medium-and high dose Astragalus polysaccharide groups,with 15 mice in each group.The low-medium-and high-dose groups of Astragalus polysaccharides were given 200、400、and 800 mg·kg^(-1)of Astragalus polysaccharides per day,respectively,and the selenium preparation group was given 0.26 mg/kg^(-1)sodium selenite tablets per day,and the blank control group and the model agent group were given the same volume of distilled water per day for 8 weeks.The serum levels of interleukin-6(IL-6),interleukin-10(IL-10),interleukin-12(IL-12),interleukin-17(IL-17),transforming growth factorβ1(TGF-β1),anti-thyroglobulin antibody(TgAb),thyroid-stimulating hormone(TSH)levels in the serum of mice in each group were detected.HE staining was used to observe the thyroid lymphocyte infiltration in each group.Results Compared with the blank control group,the serum levels of IL-6,IL-12,IL-17 and TgAb were significantly increased in the model group,while those in the other treatment groups were significantly decreased(P<0.001).Compared with the blank control group,the serum levels of IL-10 and TGF-β1 in the model group decreased significantly,while those in the other treatment groups increased,and the difference was statistically significant(P<0.001).Compared with the blank control group,the serum TSH of mice in the other groups was increased,but there was no significant difference between the groups,which confirmed the non-hyperthyroidism and hypothyroidism.HE staining showed that the thyroid lymphocyte infiltration of mice in the AIT group was significant,and the thyroid lymphocyte infiltration in each treatment group was restore

关 键 词：自身免疫性甲状腺炎 黄芪多糖 炎症因子 免疫功能 NOD.H-2^(h4)小鼠 甲状腺形态学 

分 类 号：R285.5[医药卫生—中药学]