Genome-editing of a circadian clock gene TaPRR95 facilitates wheat peduncle growth and heading date  

作　　者：Mingxue Fu Shaoshuai Liu Yuqing Che Dada Cui Zhongyin Deng Yang Li Xinyu Zou Xingchen Kong Guoliang Chen Min Zhang Yifan Liu Xiang Wang Wei Liu Danmei Liu Shuaifeng Geng Aili Li Long Mao 

机构地区：[1]State Key Laboratory of Crop Gene Resources and Breeding and National Key Facility for Crop Resources and Genetic Improvement,Institute of Crop Science,ChineseAcademyofAgricultural Sciences,Beijing 100081,China [2]College of Life Sciences,Henan Normal University,Xinxiang,Henan 453007,China [3]College of Agronomy,Sichuan Agricultural University,Chengdu,Sichuan 610106,China [4]College of Agronomy,Henan Agricultural University,Zhengzhou,Henan 450002,China [5]School of Life Science,Shanxi University,Taiyuan,Shanxi 030006,China

出　　处：《Journal of Genetics and Genomics》2024年第10期1101-1110,共10页遗传学报（英文版）

基　　金：We are grateful for the funding from STI 2030-Major Projects(2023ZD0406802);the National Natural Science Foundation of China(32072066,32172050,3220151460);Hainan Yazhou Bay Seed Lab(B21HJ0215);CAAS Agricultural Science and Technology Innovation Program(CAAS-ZDRW202002,CAAS-ZDRW202201);Hebei Natural Science Foundation(C2021205013).

摘　　要：Plant height and heading date are important agronomic traits in wheat(Triticum aestivum L.)that affect final grain yield.In wheat,knowledge of pseudo-response regulator(PRR)genes on agronomic traits is limited.Here,we identify a wheat TaPRR95 gene by genome-wide association studies to be associated with plant height.Triple allele mutant plants produced by CRISPR/Cas9 show increased plant height,particularly the peduncle,with an earlier heading date.The longer peduncle is mainly caused by the increased cell elon-gation at its upper section,whilst the early heading date is accompanied by elevated expression of flow-ering genes,such as TaFT and TacO1.A peduncle-specific transcriptome analysis reveals up-regulated photosynthesis genes and down-regulated IAAVAux genes for auxin signaling inpr95abad plants that may act as a regulatory mechanism to promote robust plant growth.A haplotype analysis identifies a TaPRR95-B haplotype(Hap2)to be closely associated with reduced plant height and increased thousand-grain weight.Moreover,the Hap2 frequency is higher in cultivars than that in landraces,suggesting the artifi-cial selection on the allele during wheat breeding.These findings suggest that TaPRR95 is a regulator for plant height and heading date,thereby providing an important target for wheat yield improvement.

关 键 词：Pseudo-response regulator(PRR) TaPRR95 CRISPR/Cas9 Plant height Heading date Photosynthesis PHYTOHORMONE WHEAT 

分 类 号：S512.1[农业科学—作物学]