蓖麻油酸12-羟化酶基因启动子克隆与分析  

Cloning and Analysis of the Acid 12-Hydroxylase Promoter in Castor

作　　者：王超 刘红光 刘婷婷 郭娇 谢凯丽 赵好 张晓明 徐刚 焦其庆[4] 谭德云 WANG Chao;LIU Hongguang;LIU Tingting;GUO Jiao;XIE Kaili;ZHAO Hao;ZHAN Xiaoming;XU Cang;JIAO Qiqing;TAN Deyun(Zibo Agricultural Sciences Research Institute,Zibo 255022,Shandong,China;Zibo Engineering Research Center for Economic Crops,Zibo 255022,Shandong,China;Crop Research Institute,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750000,Ningxia,China;Shandong Academy of Agricultural Sciences,Jinan 250131,Shandong,China)

机构地区：[1]淄博市农业科学研究院,山东淄博255022 [2]经济作物淄博市工程研究中心,山东淄博255022 [3]宁夏农林科学院农作物研究所,宁夏银川750000 [4]山东省农业科学院,山东济南250131

出　　处：《中国麻业科学》2024年第5期261-266,共6页Plant Fiber Sciences in China

基　　金：中央引导地方科技发展项目(YDZX2023021);山东省现代耕作制度技术体系淄博综合试验站(SDAIT-31-08);淄博市农业科学研究院青年人才科创项目(zbnkqn2024-02)。

摘　　要：蓖麻(Ricinus communis L.)是一种重要的工业油料作物,蓖麻油的主要成分是蓖麻油酸。蓖麻油酸12-羟化酶(oleate 12-hydroxylase, FAH12)是调控蓖麻油酸合成的关键酶,且仅在蓖麻种子中高效表达,具有组织特异性表达特性。经研究发现,FAH12基因表达调控元件主要集中在基因前端-1~-1432 bp (24680984~24682416),共含有84个TATA核心启动元件,42个标准启动子调控元件CAAT-Box, 2个胚乳形成相关的特异性表达元件Skn-1。以蓖麻基因组为材料,获得蓖麻油酸12羟化酶基因启动子,克隆到pCAMBIA1305.2表达载体中,替换组成型启动子CaMV35S,命名为pCAMBIA-FA12P。研究构建的表达载体pCAMBIA-FA12P可以用于后续引导目的基因在蓖麻中特异性表达的研究。Ricinus communis L.is an important industrial oil crop,and the main component of castor oil is ricinoleic acid.Oleate 12-hydroxylase(FAH12)is a key enzyme in the regulation of ricinoleic acid biosynthesis,which is efficiently expressed only in the seeds of the castor bean,displaying characteristic tissue-specific expression.It has been found that the regulatory elements of FAH12 gene expression are mainly concentrated in the region from-1 bp to-1432 bp(24680984~24682416)upstream of the gene,including 84 TATA core promoter elements,42 standard promoter regulatory elements CAAT-Box,and 2 specific expression elements Skn-1 related to endosperm formation.Using the castor bean genome as the material,the promoter of the ricinoleic acid 12-hydroxylase gene was obtained and inserted into the pCAMBIA1305.2 expression vector,replacing the constitutive promoter CaMV35S,and named as pCAMBIA-FA12P.The expression vector pCAMBIA-FA12P constructed in this study can be used for subsequent studies on guiding the specific expression of target genes in castor bean.

关 键 词：蓖麻 油酸12-羟化酶 启动子 特异性表达 

分 类 号：S565.6[农业科学—作物学]