RAP1B对类风湿关节炎成纤维样滑膜细胞生物学行为的影响研究  

The effects of RAP1B on fibroblast-like synoviocytes in rheumatoid arthritis

作　　者：张韵格 张可悦 黄娴倩[1] 陈吉 黄佳丽 陈勇[1] ZHANG Yunge;ZHANG Keyue;HUANG Xianqian;CHEN Ji;HUANG Jiali;CHEN Yong(Department of Rheumatology,Ningbo No.2 Hospital,Ningbo 315099,China;Department of Geratology,The First Affiliated Hospital of Ningbo University Ningbo No.1 Hospital,Ningbo 315010,China;Health Science Center,Ningbo University,Ningbo 315211,China;Cixi Biomedical Research Institute,Wenzhou Medical University,Ningbo 315300,China)

机构地区：[1]宁波市第二医院风湿免疫科,浙江宁波315099 [2]宁波大学附属第一医院/宁波市第一医院老年医学科,浙江宁波315010 [3]宁波大学医学部,浙江宁波315211 [4]温州医科大学慈溪生物医药研究院,浙江宁波315300

出　　处：《中华临床免疫和变态反应杂志》2024年第5期432-438,共7页Chinese Journal of Allergy & Clinical Immunology

基　　金：浙江省医药卫生科技计划项目(2023KY1082);宁波市第二医院华美研究基金项目(2021HMKY20);宁波市自然科学基金一般项目(202003N4249);宁波市第二医院院级重点学科-风湿免疫科(2023-Y04)。

摘　　要：目的探讨RAP1B对体外培养的类风湿关节炎(rheumatoid arthritis,RA)成纤维样滑膜细胞(fibroblast-1ike synoviocyte,FLS)增殖、迁移以及炎症因子分泌能力的影响。方法通过构建RAP1B过表达及干扰表达的慢病毒载体,转染至人RA成纤维样滑膜细胞(MH7A),分别采用CCK8法、划痕实验、Transwell-迁移实验及ELISA法,对MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力进行检测。结果RAP1B表达上调后,MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力均明显增强(均P<0.01);RAP1B表达下调后,MH7A增殖、迁移及炎症因子(IL-1β、IL-6、TNF-α、IL-8)分泌能力均明显减弱(均P<0.05)。结论RAP1B具有促进RA-FLS增殖、迁移及炎症因子分泌的能力。Objective To investigate the impact of RAS-related protein 1B(RAP1B)on the proliferation,migration,and inflammatory factor secretion of fibroblast-like synoviocytes(FLS)in rheumatoid arthritis(RA).Methods We constructed lentiviral vectors for both overexpression and interference expression of RAP1B,which were then stably transfected into human RA-FLS(MH7A).The proliferation,migration,and secretion of inflammatory factors(IL-1β,IL-6,TNF-α,IL-8)of MH7A cells were detected by the CCK8 method,scratch test,Transwell-migration test,and ELISA,respectively.Results Up-regulation of RAP1B enhanced the proliferation,migration,and inflammatory factor secretion of MH7A cells,while down-regulation of RAP1B had the opposite effect.Conclusion RAP1B has the capacity to promote the proliferation,migration,and secretion of inflammatory factors(IL-1β,IL-6,TNF-α,and IL-8)in RA-FLS.

关 键 词：类风湿关节炎 成纤维样滑膜细胞 RAP1B 

分 类 号：R593.22[医药卫生—内科学]