大豆FAD2基因家族生物信息学及表达模式分析  

Bioinformatics and Expression Pattern Analysis of Soybean FAD2 Gene Family

作　　者：吴楠 叶壮 元明浩 刘明 宁夕琳 Wu Nan;Ye Zhuang;Yuan Minghao;Liu Ming;Ning Xilin(College of Agricultural Science and Technology University,Jilin,132101)

机构地区：[1]吉林农业科技学院农学院,吉林132101

出　　处：《分子植物育种》2024年第21期6986-6995,共10页Molecular Plant Breeding

基　　金：吉林农业科技学院博士启动基金项目(20200008)资助。

摘　　要：为了阐明大豆脂肪酸去饱和酶FAD2家族中7个基因的同源性和结构差异,以及每个基因在籽粒发育不同阶段的表达水平与油酸含量之间的关系,通过对该家族中的7个基因进行生物信息学和表达模式的分析。结果表明,GmFAD2-1和GmFAD2-2b等基因在结构上存在差异,推测它们的功能也有微弱的变化。通过分析大豆籽粒发育不同阶段的基因表达模式,发现大豆家族基因在籽粒发育不同时期的表达水平先升高后降低,最后降低趋于稳定性。相关分析表明,基因表达与油酸含量呈负相关,GmFAD2-1和GmFAD2-2b的相关系数最大,呈显著的负相关。分析每个基因间的结构以及大豆品种和籽粒发育不同阶段表达水平的差异,初步识别基因之间功能大小和催化效率的差异,为利用基因工程技术创造高油酸大豆新品种提供理论依据。To clarify the homology and structural differences of seven genes in the soybean fatty acid desaturase 2(FAD2)family and the relationship between the expression level of each gene and oleic acid content at different stages of grain development,the seven genes in this family were studied through informatics analysis and evaluation of expression patterns.The results showed that GmFAD2-1 and GmFAD2-2b and other genes are structurally different,and it is speculated that their functions also have weak changes.By analyzing gene expression patterns at different stages of soybean grain development,the expression levels of the family genes at different stages of grain development first increase,then decrease,and finally decrease for stability.The correlation analysis showed that the gene expression was negatively correlated with oleic acid content,and the correlation coefficients of GmFAD2-1 and GmFAD2-2b were the largest,showing a significant negative correlation.Bioinformatics and expression analysis of genes in the FAD2 family.Identify the structural differences of each gene and the differences in expression levels at different stages of soybean variety and grain development,and initially identify the differences in function size and catalytic efficiency between genes,in order to create new high-oleic soybean varieties using genetic engineering quality,providing a theoretical basis.

关 键 词：脂肪酸脱饱和酶基因 生物信息学 表达模式分析 油酸含量 

分 类 号：S565.1[农业科学—作物学]