外源CPPU抑制‘妃子笑’荔枝果实着色过程的基因表达分析  

Gene Expression Analysis of Exogenous CPPU Inhibition the Coloration Process of'Feizixiao'Litchi Fruit

作　　者：张月 岳晓琦 张付祥 杨成坤 唐丽雯 丁峰[4] 胡福初[5] 周开兵[1,2] 马武强 Zhang Yue;Yue Xiaoqi;Zhang Fuxiang;Yang Chengkun;Tang Liwen;Ding Feng;Hu Fuchu;Zhou Kaibing;Ma Wuqiang(Sanya Institute of Breeding and Multiplication,Hainan University,Sanya,572025;School of Tropical Agriculture and Forestry,Hainan University,Haikou,570228;Chagrak Township People's Government,Wensu,843108;Guangxi Crop Genetic Improvement and Biotechnology Laboratory,Guangxi Academy of Agricultural Sciences,Nanning,530007;Investigation Station of Tropical Fruit Trees(Ministry of Agriculture and Rural Affairs),Key Laboratory of Tropical Fruit Tree Biology of Hainan Province,Institute of Tropical Fruit Tree,Hainan Academy of Agricultural Sciences,Haikou,571100)

机构地区：[1]海南大学三亚南繁研究院,三亚572025 [2]海南大学热带农林学院,海口570228 [3]阿克苏地区温宿县恰格拉克乡人民政府,温宿843108 [4]广西壮族自治区农业科学院,广西作物遗传改良生物技术重点开放实验室,南宁530007 [5]海南省农业科学院热带果树研究所,海南省热带果树生物学重点实验室,农业农村部海口热带果树科学观测实验站,海口571100

出　　处：《分子植物育种》2024年第21期7004-7022,共19页Molecular Plant Breeding

基　　金：海南省基础与应用基础研究计划(自然科学领域)青年项目(320QN191);海南省重大科技计划项目(ZDKJ2021006);国家自然科学基金项目(32260750);海南大学引进高层次人才启动基金项目[KYQD(2R)1970];海南省研究生创新科研课题项目(Qhys2021-249)共同资助。

摘　　要：为了探究花青苷和叶绿素相关结构基因与‘妃子笑’荔枝果皮着色的关系,本研究首先基于基因组数据鉴定了花青苷和叶绿素相关结构基因,然后分析了其在外源CPPU抑制‘妃子笑’荔枝着色过程的表达及对应酶活性。结果显示,花青苷合成关键酶UFGT,叶绿素降解途径NYC/NOL、MDcase以及PAO酶活性明显受到抑制,叶绿素合成途径CS和CAO及Chlase酶活性明显增加。转录组测序和RT-qPCR结果表明,外源CPPU显著抑制了花青苷合成途径结构基因LcPAL1、LcPAL3、LcC4H1、Lc4CL2、Lc4CL3、Lc4CL4、LcCHS2、LcCHS5、LcCHI、LcF3H/LcF3'H、LcDFR2、LcANS1、LcUFGT2、LcUFGT3,和叶绿素降解途径结构基因LcSGR2、LcPPH和LcPAO的表达;促进叶绿素合成相关途径基因LcHEMA、LcHEMD、LcCHLH1、LcCHLH2和LcCAO的表达,同时抑制转录因子基因LcMYB3、LcMYB4和LcWRKY3的表达。因此,推测外源CPPU可能通过抑制花青苷合成和叶绿素降解相关基因表达及酶活性,促进叶绿素合成相关基因的表达及酶活性,使‘妃子笑’荔枝果皮中花青苷与叶绿素比值在最佳可食期偏低而不能完全着色。研究结果为解决‘妃子笑’荔枝“滞绿”问题提供理论依据,也可为植物色泽问题的研究提供参考。In order to explore the relationship between the anthocyanin and chlorophyll related structural genes with the coloration of'Feizixiao'litchi,identification of the above gene based on genome data was conducted firstly,and then analyzed their expression and corresponding enzyme activities during the coloration inhibition of'Feizixiao'litchi induced by exogenous CPPU in this study.The results showed that the key enzyme UFGT in anthocyanin biosynthesis,as well as the enzymes NYC/NOL,MDcase,and PAO involved in the chlorophyll degradation path way,were significantly inhibited,while the activities of CS and CAO in the chlorophyll synthesis pathway,along with Chlase,were notably increased.Transcriptome sequencing and RT-qPCR results indicated that exogenous CPPU significantly inhibited the expression of structural genes involved in the anthocyanin biosynthesis pathway,including LcPAL1,LcPAL3,LcC4H1,Lc4CL2,Lc4CL3,Lc4CL4,LcCHS2,LcCHS5,LcCHI,LcF3H/LcF3'H,LcDFR2,LcANS1,LcUFGT2,LcUFGT3,and those involved in the chlorophyll degradation pathway,including LcSGR2,LcPPH,and LcPAO.At the same time,it promoted the expression of genes related to chlorophyll synthesis,such as LcHEMA,LcHEMD,LcCHLH1,LcCHLH2 and LcCAO,while inhibiting the expression of transcription factor genes LcMYB3,LcMYB4 and LcWRKY3.Therefore,it is speculated the expression and enzyme activity of genes related to anthocyanin synthesis and chlorophyll degradation may be inhibited,the expression and enzyme activity of genes related to chlorophyll synthesis may be promoted by exogenous CPPU to make the ratio of anthocyanin to chlorophyll in the pericarp of'Feizixiao'litchi fruit lower in the best edible period and unable to complete coloration.The results provide a theoretical basis for solving the problem of"stay green"in'Feizixiao'litchi,and also provide a reference for the study of plant color.

关 键 词：荔枝(Litchi chinensis Sonn.) CPPU 着色 酶活性 基因表达 

分 类 号：S667.1[农业科学—果树学]