基于单分子实时测序的金花茶全长转录组分析  

Full-length Transcriptome Analysis of Camellia nitidissima Based on Single-Molecule Real-Time Sequencing Technology

作　　者：刘合霞 姚金双 刘雨 谢心怡 耿晓姗 李博 周兴文 Liu Hexia;Yao Jinshuang;Liu Yu;Xie Xinyi;Geng Xiaoshan;Li Bo;Zhou Xingwen(College of Biology and Pharmacy of Yulin Normal University,Yulin,537000;College of Architecture and Planning,Fujian University of Technology,Fuzhou,350108)

机构地区：[1]玉林师范学院生物与制药学院,玉林537000 [2]福建工程学院建筑与城乡规划学院,福州350108

出　　处：《分子植物育种》2024年第21期7031-7039,共9页Molecular Plant Breeding

基　　金：玉林师范学院高层次人才科研启动基金项目(G2018025,G2019ZK13,G2019ZK35);玉林师范学院大学生创新创业训练计划项目(202010606164);玉林市科学研究与技术开发计划项目(玉市科能20194303);广西高校中青年教师科研基础能力提升项目(2020KY14012);广西自然科学基金重点项目(2018GXNSFDA281007);国家自然科学基金项目(31860228)共同资助。

摘　　要：金花茶是中国特有植物,是培养黄色山茶花的珍贵种质资源,具有重要的观赏价值和药用价值。金花茶全长转录组测序数据有助于筛选出调控花色合成的基因,但是金花茶的全长转录组数据仍然缺乏。因此,本研究利用单分子实时测序技术对金花茶花芽、花蕾以及盛开的花进行混合样品的全长转录组测序。测序结果表明,在28.2 Gb的原始测序数据中,共生成了179645个全长非嵌合序列(FLNC),从中获得了45372个高质量的全长转录本(Isoform)。对获得的全长转录本进行可变剪切分析及长链非编码RNA预测,鉴定出了7752个可变剪接事件和1730个长链非编码RNA序列;在基因注释分析中,NR、Swiss-Prot、KOG和KEGG数据库分别注释了43348、36909、29452和42163条转录本,检测到了1787个转录因子。GO注释及KEGG代谢通路分析表明,金花茶的花芽、花蕾、开放花朵等组织的基因表达谱主要与次生代谢物质合成、细胞功能以及细胞组分合成有关。本研究提供了大量的全长转录本序列,为今后金花茶的分子生物学和遗传学研究奠定了基础。Camellia nitidissima is endemic plant in China.It is precious germplasm resource for cultivating yellow camellia,which has important ornamental value and medicinal value.Full-length transcriptome sequencing data of Camellia nitidissima can help to find genes which regulate the synthesis of flower color,but the full-length Camellia nitidissima transcriptome is still lacking.So the alabastrum,flower bud and blooming flowers mixed equally were sequenced using single-molecule real-time sequencing technology to generate the transcriptome of Camellia nitidissima.The sequencing results showed that 179645 full-length non-contatemer(FLNC)were symbiotic in 28.2 Gb raw data,from which 45372 high-quality Isoforms were obtained.7752 alternative splicing events and 1730 long non-coding RNA sequences were identified through prediction of alternative splicing and long non-coding RNA.43348,36909,29452 and 42163 Isoforms were respectively assigned to the NR,Swiss-Prot,KOG and KEGG databases in gene annotation analysis,and 1787 transcription factors were detected.The results of Gene Ontology annotation and KEGG analysis indicated the gene expression profile of alabastrum,flower bud and blooming flowers was mainly related to the synthesis of secondary metabolites,cell functions and cell components.This study provided a large number of full-length transcript sequences,which will lay a foundation for the molecular biology and genetics of Camellia nitidissima in the future.

关 键 词：金花茶 单分子实时测序 可变剪切 长非编码RNA 

分 类 号：S685.14[农业科学—观赏园艺]