青檀对不同氮素水平响应过程中的长链非编码RNA鉴定  

Identification of Long Non-coding RNAs of Pteroceltis tatarinowii in Response to Different Nitrogen Application Levels

作　　者：郭伟 李国华[1] 罗磊[1] 王迎[1] 张利 程甜甜[1] 周光锋[1] 杨波[1] 穆淑媛 季霞[1] 边晓惠 孙忠奎 于永畅 李冬梅[1] 韩秀 付均惠 王新花[1] 张林[1] Guo Wei;Li Guohua;Luo Lei;Wang Ying;Zhang Li;Cheng Tiantian;Zhou Guangfeng;Yang Bo;Mu Shuyuan;Ji Xia;Bian Xiaohui;Sun Zhongkui;Yu Yongchang;Li Dongmei;Han Xiu;Fu Junhui;Wang Xinhua;Zhang Lin(Taishan Academy of Forestry Sciences,Tai'an City,Taian,271000;College of Agricultural and Biological Engineering,Heze University,Heze,274015;Taian Time Garden Technology Development Co.,Ltd.,Taian,271000;Taishan University,Taian,271021)

机构地区：[1]泰安市泰山林业科学研究院,泰安271000 [2]菏泽学院农业与生物工程学院,菏泽274015 [3]泰安时代园林科技开发有限公司,泰安271000 [4]泰山学院,泰安271021

出　　处：《分子植物育种》2024年第21期7040-7045,共6页Molecular Plant Breeding

基　　金：泰安市乡土观赏树种国家林木种质资源库项目(林场发[2016]153号);山东省农业科技园区产业提升工程项目(2019YQ012);泰安市科技发展计划(引导计划)项目(2018NS0090)共同资助。

摘　　要：青檀(Pteroceltis tatarinowii)是耐干旱瘠薄的石灰岩山地造林先锋树种,其韧皮纤维是制作“宣纸”的原材料。本研究采用PLEK、CNCI、CPC2、Pfam 4个生物信息学软件对青檀氮素响应全长转录组得到的11801条基因序列进行蛋白编码潜能预测分析,分别获得5077、3299、2202、2878个长链非编码RNA(Long noncoding RNA),通过制作韦恩图取4组数据的交集,最终获得1161个lncRNA,最短的为200 nt,最长的为4122 nt,大部分lncRNA长度在200~1000 nt区间,占总数的92.33%。本研究结果为深入开展青檀氮素响应分子机制的研究提供了参考。Pteroceltis tatarinowii being successfully employed as a pioneer tree species in restoration projects in limestone mountain,has phloem fibers that are used as the main material to manufacture the world famous Xuan paper.Four bioinformatics softwares,PLEK,CNCI,CPC2,Pfam,were employed to predict protein-coding potential for the 11801 genes generated based on the SMRT-seq data.Finally 5077,3299,2202,2878 long non-coding RNAs(lncRNAs)were obtained with the four bioinformatics softwares,respectively,while 1161lncRNAs with minimum length of 200 nt and maximum length of 4122 nt were gained from the intersection of the four groups of data by making Venn diagram.Most of the lncRNAs,92.33% of the total,have length between 200and 1000 nt.The results of the study provide a reference for further research on the molecular mechanism of nitrogen response of P.tatarinowii.

关 键 词：青檀(Pteroceltis tatarinowii) 全长转录组 lncRNA 

分 类 号：S792.99[农业科学—林木遗传育种]