北苍术有效成分积累对干旱胁迫的响应及其机制探究  

Exploration of the Response and Mechanism of Effective Component Accumulation in Atractylodes chinensis to Drought Stress

作　　者：杨雪晴 徐伟[1] 肖春萍 孙金 翁丽丽[1] 冯禹壮 Yang Xueqing;Xu Wei;Xiao Chunping;Sun Jin;Weng Lili;Feng Yuzhuang(College of Pharmacy,Changchun University of Traditional Chinese Medicine,Changchun,130117)

机构地区：[1]长春中医药大学药学院,长春130117

出　　处：《分子植物育种》2024年第21期7150-7158,共9页Molecular Plant Breeding

基　　金：国家自然科学基金项目(81803649);吉林省科技厅科技项目(20191102035YY);吉林省中医药管理局科技项目(2020047);吉林省教育厅“十三五”科技厅项目(JJKH20190477KJ)共同资助。

摘　　要：本研究通过人工模拟降雨,对一年生盆栽北苍术进行长期和短期干旱胁迫研究;采用高效液相色谱法测定不同干旱胁迫方式下北苍术根茎中苍术素的含量;通过双抗体夹心法测定不同干旱胁迫方式对苍术素合成关键酶ACC活性的影响;利用实时荧光定量PCR(RT-qPCR)法测定不同干旱胁迫方式下关键酶ACC基因表达量。结果表明,生殖生长期内轻、中度干旱胁迫下苍术素积累显著,苍术素含量分别比CK提高9.67%、10.63%(长期),另外在短期干旱胁迫处理12 d时苍术素含量达到峰值,比对照组提高6.36%,重度干旱胁迫显著抑制了苍术素的合成;长期的轻、中度和短期干旱胁迫有利于显著提高ACC的酶活性和基因表达量(P<0.05),长期重度干旱胁迫抑制苍术素生物合成途径中ACC酶活性及其表达,分别较CK显著下降12.08%和48.66%,进而显著抑制苍术素合成。该研究对实现北苍术药材质量调控具有重要的理论价值。In this study,artificial rainfall simulation was used to study the long-term and short-term drought stress of annual potted Atractylodes chinensis;the content of atractylodin in rhizomes of Atractylodes chinensis under different drought stress was determined by high performance liquid chromatography;the effects of different drought stress on the activity of ACC,the key enzyme of atractylodin synthesis,were measured by double antibody sandwich method;the gene expression of key enzyme ACC under different drought stress was determined by real-time fluorescence quantitative PCR(RT-qPCR).The results showed that during the reproductive growth period,atractylodin accumulated significantly under light and moderate drought stress,and the content of atractylodes was higher than CK increased by 9.67% and 10.63%(long-term),in the short-term drought stress treatment 12 d atractylodin content of peak,6.36% higher than that of CK.Severe drought stress significantly inhibited the synthesis of atractylodin.Long-term mild,moderate and short-term drought stress was beneficial to significantly increase the enzyme activity and gene expression of ACC(P<0.05).Long term severe drought stress inhibited the enzyme activity and expression of ACC in atractylodin biosynthesis pathway,which decreased by 12.08% and 48.66% respectively compared with CK,and then significantly inhibited atractylodin synthesis.This study has important theoretical value for the quality control of Atractylodes chinensis.

关 键 词：北苍术 干旱胁迫 苍术素 关键酶 基因表达量 

分 类 号：S567.239[农业科学—中草药栽培]