草珊瑚再生体系建立及繁殖分析  

Establishment and Propagation Analysis of Grass Coral Regeneration System

作　　者：戴凌 何荣晓[2] 王友雄 何碧珠[3] 郭梨锦 Dai Ling;He Rongxiao;Wang Youxiong;He Bizhu;Guo Lijin(Hainan Xinlvgshen Tropical Bioengineering Co.,Ltd.,Haikou,570100;College of Forestry,Hainan University,Haikou,570228;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,350000;International Magnesium Nutrition Institute,College of Resource and Environment,Fujian Agriculture and Forestry University,Fuzhou,350000)

机构地区：[1]海南新绿神热带生物工程有限责任公司,海口570100 [2]海南大学林学院,海口570228 [3]福建农林大学园艺学院,福州350000 [4]福建农林大学资源与环境学院,国际镁营养研究所,福州350000

出　　处：《分子植物育种》2024年第21期7166-7173,共8页Molecular Plant Breeding

基　　金：五指山生态科技特派员项目(KH200108A)资助。

摘　　要：为了建立草珊瑚再生体系,加快其繁殖速度,缩短繁育周期,为草珊瑚的组培工厂化育苗和产业化发展利用提供一定的参考,本研究以草珊瑚茎段为外植体,通过控制外植体取材时间,消毒试剂类型及消毒时长,培养基类型以及外源激素浓度配比的不同,从初代培养到继代培养再到生芽、壮苗与生根和试管苗移栽,以此获得最佳培养条件。结果表明:草珊瑚外植体采摘最佳时间为3~5月;腋芽茎段消毒以2%NaClO处理14 min或0.1%HgCl2消毒12 min达到最佳消毒效果;芽诱导培养基为MS+1.0 mg/L 6-BA+0.1 mg/L NAA+2.5%蔗糖+1.0 g/L活性炭+0.7%琼脂,萌芽率可达85%;芽增殖培养基为MS+1.0 mg/L 6-BA+2.5%蔗糖+1.0 g/L活性炭+0.7%琼脂,增殖系数可达5.9;生根诱导培养基为1/2MS+1.0 mg/L IBA+0.1 mg/L NAA+2.5%蔗糖+1.0 g/L活性炭+0.7%琼脂,生根率达到95%,根粗长,芽苗形态长势好;草珊瑚小苗移栽以砂子:腐殖土1:1为基质成活率最高。本研究建立草珊瑚的无菌再生体系,为草珊瑚的工业化生产提供一定的参考。In order to establish the regeneration system of grass coral(Sarcandra glabra),speed up its reproduction rate,shorten the breeding cycle,and provide certain reference for the tissue culture and industrial development and utilization of grass coral,this study took grass coral stem segment as explants and controlled the sampling time of explants,the types and duration of disinfection reagents,the types of culture medium and the concentration ratio of exogenous hormones.From primary culture to secondary culture to sprout,strong seedling and root and tube seedling transplanting,so as to obtain the best culture conditions.The results showed that the best time to pick the explants was from March to May.Axillary bud stem segments were treated with 2% NaClO for 14 minutes or 0.1% HgCl_(2) for 12 minutes to achieve the best disinfection effect.The bud induction medium was MS+1.0 mg/L6-BA+0.1 mg/L NAA+2.5% sucrose+1.0 g/L activated carbon+0.7% AGAR,and the germination rate could reach85%.The proliferation medium was MS+1.0 mg/L 6-BA+2.5% sucrose+1.0 g/L activated carbon+0.7% AGAR,and the proliferation coefficient could reach 5.9.The root induction medium was 1/2MS+1.0 mg/L IBA+0.1 mg/L NAA+2.5%sucrose+1.0 g/L activated carbon+0.7% AGAR,the rooting rate reached 95%.The survival rate of grass coral seedlings was the highest when sand:humus 1:1 was used as the substrate.The aseptic regeneration system established in this study can provide some reference for the industrial production of grass coral.

关 键 词：草珊瑚 丛生芽 初代诱导 继代增殖 生根诱导 

分 类 号：S567.239[农业科学—中草药栽培]