犬源细小病毒兰州株的分离鉴定与VP2基因的遗传进化分析  

Isolation,Identification of Parvovirus from Canine in Lanzhou and Phylogeny Analysis of VP2 Gene Sequences

作　　者：孔繁丽 卢曾军[2] 陈慧丽 李坤[2] 刘果 张小丽[1] KONG Fanli;LU Zengjun;CHEN Huili;LI Kun;LIU Guo;ZHANG Xiaoli(College of Animal Medicine,Gansu Agricultural University,Lanzhou 730070,China;Lanzhou Institute of Veterinary Medicine,Chinese Academy of Agricultural Sciences,Lanzhou 730064,China;Linxia Agricultural and Rural Bureau,Linxia Gansu 731199,China)

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]中国农业科学院兰州兽医研究所/兰州大学动物医学与生物安全学院家畜疫病病原生物学国家重点实验室,兰州730064 [3]甘肃省临夏市农业农村局,甘肃临夏731199

出　　处：《西北农业学报》2024年第11期2011-2019,共9页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：甘肃农业大学公招博士科研启动基金(GAU-KYQD-2021-221010);甘肃省高等学校创新基金(2022A-049);甘肃省科协创新驱动助力工程项目(GXH20230817-4)。

摘　　要：为了解近年来甘肃省兰州市犬细小病毒(Canine parvovirus,CPV)的基因型分布情况及遗传进化方向,于兰州市宠物医院采集8份细小病毒阳性(试纸条检测)的犬粪便样品,使用F81细胞分离病毒;利用PCR、间接免疫荧光试验、血凝试验鉴定所分离的病毒;随后分析病毒基因型和VP2基因的遗传进化关系。结果显示,共分离得到7株细小病毒,1株为New CPV-2a型CPV,5株为CPV-2c型CPV,1株为猫细小病毒(Feline parvovirus,FPV)。VP2氨基酸序列同源性及进化分析结果显示,分离到的CPV-2c型毒株VP2同源性为100%,与北京、江苏等地分离株的亲缘关系较近;New CPV-2a型毒株与吉林、西安等地的分离株亲缘关系较近;犬源猫细小病毒(LZ05)与已报道的FPV毒株相比,存在91S→A、322V→T独特的突变,且与2020年北京一株犬源猫细小病毒分离株亲缘关系最近。To investigate the genotype distribution and genetic evolution of canine parvovirus(CPV)in Lanzhou in recent years,eight fecal samples tested positive using a canine parvovirus rapid test kit were collected from pet hospitals in Lanzhou.F81 cells were used for isolating parvovirus strains;PCR,indirect immunofluorescence and hemagglutination assays were conducted to identify the isolated strains;additionally,the genotype and genetic evolution relationship were analyzed.The results showed that seven parvovirus strains were isolated,one strain belonged to the New CPV-2a type,five strains were classified as CPV-2c type,and one strain was identified as Feline parvovirus(FPV).The homology of amino acid sequences and evolutionary analysis showed that the VP2 sequences of these CPV-2c strain was 100%homologous and closely related to the strains found in Beijing and Jiangsu.The New CPV-2a strain exhibited similarities to those found in Jilin and Xi’an.Compared with the reported strains,FPV of canine origin(LZ05)had unique mutations(91S→A and 322V→T),and was found to be most closely related to a strain isolated in Beijing in 2020.

关 键 词：犬细小病毒 分离鉴定 VP2 遗传进化分析 

分 类 号：S852.65[农业科学—基础兽医学]