基于Sanger测序技术的云南荔枝园蠹虫种类初步鉴定  

Preliminary Identification of Beetle Species in Litchi Orchards in Yunnan Based on Sanger Sequencing Technology

作　　者：宋云连 罗心平[1] 章勇 毕珏 赵一 张惠云[1] SONG Yunlian;LUO Xinping;ZHANG Yong;BI Jue;ZHAO Yi;ZHANG Huiyun(Institute of Tropical and Subtropical Economic Crops,Yunnan Academy of Agricultural Sciences,Baoshan,Yunnan 678000,China;Yunnan Rural Science and Technology Service Center,Kunming,Yunnan 650021,China)

机构地区：[1]云南省农业科学院热带亚热带经济作物研究所,云南保山678000 [2]云南省农村科技服务中心,云南昆明650021

出　　处：《热带农业科学》2024年第8期14-19,共6页Chinese Journal of Tropical Agriculture

基　　金：云南省创新引导与科技型企业培育计划“云南省屏边苗族自治县荔枝产业科技特派团”(No.202204BI090021);国家重点研发计划“元阳热带亚热带水果产业提质增效关键技术集成与示范”(No.2022YFD1601806);国家现代农业产业技术体系资助项目“国家荔枝龙眼产业技术体系”(No.CARS-32)。

摘　　要：荔枝作为云南乡村振兴重要产业之一,随着面积的不断扩大,其种植生态环境多样化,病虫害的种类也随之增加。近几年在云南荔枝主要产区陆续发现蠹虫为害,调查荔枝蠹虫为害情况并鉴定其种类,对云南荔枝病虫害防治具有重要意义。采用5点取样法,针对云南荔枝5个主要产区蠹虫为害程度以及为害状进行调查,利用Sanger测序技术进行生理小种初步鉴定。结果发现,5个产区均有不同程度蠹虫为害,为害状与长蠹科昆虫为害状相似。经NCBI数据库比对,初步将本次调查的蠹虫鉴定为2属3种,分别是双棘长蠹属侧突双棘长蠹(Sinotylon perforans)、双棘长蠹(Sinoxylon anale)和长蠹属槲长蠹(Bostrichus capucinus)。As one of the crucial industries for rural revitalization in Yunnan Province,litchi has diversified its planting eco-logical environment with the continuous expansion of its area,and the types of diseases and pests have also increased.Beetles have been discovered in the main production areas of litchis in Yunnan in recent years.Investigating the harm caused by litchi beetles and identifying their types is highly important for the prevention and control of lychee diseases and pests in Yunnan.This study adopted a 5-point sampling method.First,a survey was conducted on the degree and status of damage caused by beetles in the five main production areas of litchi in Yunnan.Sanger sequencing technology was used for preliminary identifi-cation of physiological races.There were varying degrees of damage caused by beetles in all five production areas,and the damage pattern was similar to that of insects in the family Scorpaenidae.After Sanger sequencing technology was used for detection,a preliminary identified through comparison in the NCBI database revealed that there are two genera and three spe-cies,namely Sinotylon perferans,Sinoxylon manage,and Bostrichus capuchinus.

关 键 词：荔枝 Sanger测序 双棘长蠹 侧突双棘长蠹 病虫害防治 

分 类 号：S436.67[农业科学—农业昆虫与害虫防治]