基于网络药理学研究灯盏细辛治疗糖尿病肾病的作用机制  

The Mechanism of the Treatment of Diabetic Nephropathy with Breviscapus was Studied Based on Network Pharmacology

作　　者：王珏 张晶 肖曼[2] 谢毅强[2] WANG Jue;ZHANG Jing;XIAO Man;XIE Yiqiang(Guizhou University of Traditional Chinese Medicine,Guiyang,Guizhou 550001,China;Hainan Medical College,Haikou,Hainan 570105,China)

机构地区：[1]贵州中医药大学,贵州贵阳550001 [2]海南医学院,海南海口570105

出　　处：《热带农业科学》2024年第8期81-90,共10页Chinese Journal of Tropical Agriculture

基　　金：海南医学院研究生创新科研课题(No.Qhys2022-304)。

摘　　要：利用网络药理学研究中草药灯盏细辛治疗糖尿病肾病(Diabetic Nephropathy,DN)的作用机制。通过TCMSP平台、ETCM数据库、PubChem数据库筛选出灯盏细辛的有效活性成分及作用靶点;利用DisGenet及GeneCards获取与DN相关靶点基因;筛选灯盏细辛活性成分作用靶点与疾病靶点的共同靶点基因。运用Cytoscape 3.9.1软件构建“成分-靶点-疾病”网络图;借助STRING在线数据库构建蛋白互作(PPI)网络图,使用Cytoscape 3.9.1软件插件ClueGo对共同靶点进行KEGG及GO分析,筛选出潜在的信号通路,并分析其作用机制。通过筛选得到灯盏细辛的有效活性成分9个,其作用于DN的靶点为79个。GO富集分析结果示,灯盏细辛-糖尿病肾病交集基因的生物功能主要涉及跨膜受体蛋白酪氨酸激酶信号通路正调控、细胞增殖、细胞凋亡过程的负调控等生物学过程。KEGG通路富集结果显示,交集基因主要分布在PI3K-AKT信号通路上。灯盏细辛的作用机制呈现多成分、多靶点、多通路的特点,本研究从药理作用及网络药理学两个方面研究灯盏细辛治疗DN的作用机制,有利于更加深入全面地了解灯盏细辛的药用价值,为其开发和利用提供理论参考。To study the mechanism of Chinese herbal medicine Calendula breviscapus in the diabetic nephropathy(DN)treatment via network pharmacology,through the TCMSP platform,ETCM database,and PubChem database,the active in-gredients and targets of Erigeron brevis were screened.Target genes related to DN were obtained via DisGenet and GeneCards.To screen the common target genes of the active ingredients of Erigeron breviscapus and the disease targets,we constructed a“component-target-disease”network diagram via Cytoscape 3.9.1 software.The protein interaction(PPI)network map was built via the STRING online database.KEGG and GO analyses of common targets were performed via the Cytoscape 3.9.1 software plug-in ClueGo to screen potential signaling pathways and analyze their mechanisms of action.Nine active ingredients of E.breviscapus were obtained through screening,and 79 targets of its action on DN were identified.GO en-richment analysis revealed that the biological functions of the genes associated with the interaction between breviscapus and diabetic nephropathy were related mainly to the positive regulation of the transmembrane receptor protein tyrosine kinase signaling pathway and the negative regulation of cell proliferation and apoptosis.The enrichment results of the KEGG path-way analysis revealed that the intersecting genes were distributed mainly in the PI3K-AKT signaling pathway.The Chinese herbal medicine E.breviscapus is a species of the Compositae family.It has the functions of dispelling wind and dampness,dispelling cold and dissolving surfaces,clearing collars,and relieving pain.Its mechanism of action shows the characteristics of multi-component,multi-target,and multi-pathway,and it is often used as a drug to prevent and treat various diseases in the clinic.This study investigated the mechanism of action of E.breviscapus in the treatment of DN from two aspects of the pharmacological action and network pharmacology,which is conducive to a more in-depth and comprehensive understanding of the medicinal valu

关 键 词：网络药理学 灯盏细辛 糖尿病肾病 作用机制 

分 类 号：S567[农业科学—中草药栽培]