探讨丹芪精颗粒对急性高原暴露下肺血管内皮的保护作用  

To investigate the protective effect of Danqijing Granule on pulmonary vascularendothelium under acute high altitude exposure

作　　者：齐小荣 陆柏辰 王亮[1] 潘宇 秦丽[1] 李敏[1] Qi Xiaorong;Lu Baichen;Wang Liang(Department of Traditional Chinese Medicine,Sixth Medical Center of PLA Gemeral Hospital,Beijing 100048,China)

机构地区：[1]解放军总医院第六医学中心中医医学部,北京100048 [2]解放军66188部队卫生连

出　　处：《中华保健医学杂志》2024年第5期601-605,共5页Chinese Journal of Health Care and Medicine

基　　金：国家自然科学基金面上项目(82274613);军委后勤保障部中医药服务能力培育与提升专项计划(2021ZY047)。

摘　　要：目的运用网络药理学结合临床试验探究阐明丹芪精颗粒对急性高原暴露后肺血管内皮的保护作用。方法首先利用中药系统药理数据库及分析平台(TCMSP)平台检索丹芪精颗粒的有效成分和药物作用靶点,并检索疾病作用靶点,获得疾病与药物的相互作用靶点。随后进行疾病和药物交叉靶点的基因本体论(GO)富集和京都基因与基因组百科全书(KEGG)通路分析,最后构建药物-疾病通路靶标网络图。根据随机对照法将80例患者分为红景天胶囊对照组和丹芪精颗粒组,各40例。分别于两组研究对象不同时间点(进驻高原后第1、3和7天)记录血清血管内皮生长因子(VEGF)和内皮素-1(ET-1)水平。结果网络药理学分析结果显示,丹芪精颗粒可能通过调节急性高原暴露中的VEGF信号通路参与炎症反应等生物学过程。临床试验结果显示,与红景天胶囊组相比,丹芪精颗粒组的VEGF水平升高(F=8.977,P<0.05),ET-1水平降低(F=5.248,P<0.05)。结论丹芪精颗粒对急性高海拔暴露具有多靶点、多通路的治疗作用,并且可以提高急性高海拔暴露患者血管内皮功能核心靶点VEGF水平、降低ET-1动态水平,对肺血管内皮细胞起保护作用。Objective To explore the protective effect of Danqijing granules on pulmonary vascular endothelium after acute exposure to high altitude by network pharmacological methods and clinical trials.Methods The active ingredients and drug targets of Danqijing granules were collected by TCMSP platform,and the targets of disease action were retrieved to obtain the targets of diseasedrug interaction.GO enrichment and KEGG pathway analysis of disease and drug crossover targets were then performed.Finally,the target network diagram of drug-disease pathway was constructed.The 80 patients were randomly divided into Rhodiola capsule control group(40 cases)and Danqijing granule group(40 cases).The levels of VEGF and ET-1 were recorded on the 1st,3rd and 7th day after the two groups were stationed at the plateau.Results The network pharmacological analysis showed that Danqijing granules may participate in the biological processes such as inflammation by regulating the VEGF signaling pathway in acute altitude exposure.The clinical trial results showed that compared with Rhodiola Rosea capsule group,the content of VEGF in Danqijing granule group was significantly increased(F=8.977,P<0.05),and the content of ET-1 was significantly decreased(F=5.248,P<0.05).Conclusion Danqijing Granule has a multi-target and multi-pathway therapeutic effect on acute high-altitude exposure,and can improve the dynamic levels of VEGF and ET-1,the core targets of vascular endothelial function,and protect pulmonary vascular endothelial cells.

关 键 词：丹芪精颗粒 急性高原暴露 网络药理学 血管内皮生长因子 内皮素-1 肺血管内皮 

分 类 号：R54[医药卫生—心血管疾病]