细菌中L-甲硫氨酸生物合成和调控机制  

L-methionine Biosynthesis and Regulatory Mechanisms in Bacteria

作　　者：崔莹 宋凯 何亚文[1] CUI Ying;SONG Kai;HE Yawen(State Key Laboratory of Microbial Metabolism,Joint International Research Laboratory of Metabolic and Developmental Sciences,School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,China)

机构地区：[1]上海交通大学生命科学技术学院,微生物代谢国家重点实验室,代谢与发育科学国际联合合作实验室,上海200240

出　　处：《激光生物学报》2024年第5期408-417,480,共11页Acta Laser Biology Sinica

基　　金：国家自然科学基金项目(31972231,32172355);上海交通大学与先正达有限公司博士生联合培养项目。

摘　　要：L-甲硫氨酸是一种必需氨基酸,在食品、饲料、化妆品和药品中具有广泛的应用。目前,L-甲硫氨酸是唯一无法用微生物发酵法工业化生产的必需氨基酸,近年来,利用代谢工程提升L-甲硫氨酸产量受到国内外研究人员的普遍重视。本文主要分析了细菌中L-甲硫氨酸的生物合成途径及调控机制,着重分析了从高丝氨酸生物合成甲硫氨酸的三个关键步骤(即酰化、硫化和甲基化),并进一步对L-甲硫氨酸的生物合成提出展望,以期为L-甲硫氨酸的工业化生产提供指导。L-methionine is an essential amino acid with a wide range of applications in food,feed,cosmetics and pharmaceuticals.Currently,L-methionine is the only essential amino acid that cannot be industrially produced by microbial fermentation.In recent years,the use of metabolic engineering to enhance the yield of L-methionine has received widespread attention from researchers at home and abroad.In this paper,the biosynthetic pathway and regulatory mechanism of L-methionine in Corynebacterium glutamicum and Escherichia coli are analysed;three key steps(i.e.,acylation,sulfurylation,and methylation)in the biosynthesis of methionine from hyper-serine are highlighted,and a further outlook on the biosynthesis of L-methionine is proposed with a view to providing guidance for the industrial production of L-methionine.

关 键 词：L-甲硫氨酸 生物合成 调控机制 

分 类 号：Q81[生物学—生物工程]