细胞内单链DNA及高通量分析技术  

Intracellular Single Strand DNA and High-Throughput Analysis Techniques

作　　者：李锐奇 赖玮毅[1] 汪海林[1,2] Ruiqi Li;Weiyi Lai;Hailin Wang(Research Center for Eco-Environmental Sciences,Chinese Academy of Sciences,Beijing 100085,China;Hangzhou Institute for Advance Study,University of Chinese Academy of Sciences,Hangzhou 310024,China)

机构地区：[1]中国科学院生态环境研究中心,北京100085 [2]国科大杭州高等研究院,杭州310024

出　　处：《化学进展》2024年第9期1283-1290,共8页Progress in Chemistry

基　　金：国家自然科学基金项目(No.22234008,21927807,22021003,22274166)资助。

摘　　要：在DNA复制、转录、双链断裂损伤修复等生命过程中,双链DNA会暂时解旋形成单链DNA(ss DNA)。ss DNA可能影响基因组的稳定性,也可能参与构成非B型结构DNA,反过来调节和影响某些关键细胞和有机体过程。本综述简要介绍了单链DNA形成的原因、参与构成的结构及在细胞中可能产生的功能,并总结了部分单链DNA高通量分析技术,为后续单链DNA研究提供方法启示,促进单链DNA分析技术和方法的进一步发展。During many life processes such as replication,transcription,double-strand breaks repair and so on,double-stranded DNA will temporarily unwind and form single strand DNA(ssDNA).ssDNA may affect genomic stability and may also participate in the formation of non-B DNA structure,which in turn regulates and influences many key cellular processes.This review briefly describes the causes of the formation of single-stranded DNA,the structures containing single-stranded DNA and their possible functions in cells,and summarizes some high-throughput analysis techniques of single-stranded DNA,which provides the method inspiration for the subsequent ssDNA research and promotes the further development of ssDNA analysis techniques and methods.

关 键 词：单链DNA R环 G-四链体 高通量测序 

分 类 号：Q523[生物学—生物化学]