基于斑马鱼SLC17A8基因的表达特征验证其对黑色素转运的影响  

Based on expression characteristics of zebrafish SLC17A8 genes,effect on melanin transport was verified

作　　者：岳丹 陆颖 李梦飞 黄代涛 张锦标 种玉晴 YUE Dan;LU Ying;LI Meng-fei;HUANG Dai-tao;ZHANG Jin-biao;CHONG Yu-qing

机构地区：[1]玉溪农业职业技术学院动物科技学院,云南玉溪653106 [2]云南农业大学动物科学技术学院,云南昆明650201

出　　处：《饲料研究》2024年第18期81-87,共7页Feed Research

基　　金：国家自然科学基金项目(项目编号:31660634);云南省动物营养与饲料重点实验室开放基金项目(项目编号:2022YNPKLANF001)。

摘　　要：试验为了探究兰坪乌骨绵羊黑色素形成的营养代谢机理,基于课题组全基因组重测序筛选出的囊泡谷氨酸转运蛋白3(SLC17A8)基因作为候选基因,利用规律成簇间隔短回文重复序列及其相关蛋白9(CRISPR/Cas9)系统基因敲除技术对SLC17A8基因于斑马鱼上进行敲除。结果表明:与野生型斑马鱼相比,36 h和42 h的突变体斑马鱼卵黄发育异常,卵黄黑色素沉积量存在个体差异,并且36 h较42 h明显,但72 h敲除卵黄黑色素变化不明显。研究表明,SLC17A8基因在黑色素转运前期具有一定作用,但后期影响不大。The study aimed to investigate the mechanism of nutritional metabolism of melanin formation in Lanping black-boned sheep.This experiment was based on the Homo sapiens solute carrier family 17,member 8(SLC17A8)gene screened by the whole genome resequencing of the subject group as a candidate gene,and the SLC17A8 gene was knocked out on zebrafish using the CRISPR/Cas9 system knockout technology.The results showed that compared with the wildtype zebrafish,36 h and 42 h mutant zebrafish had abnormal yolk development and individual differences in the amount of yolk melanin deposition,and it was more obvious at 36 h than at 42 h.However,the yolk melanin changes were not obvious at 72 h knockout.The study indicates that this gene has a role in the pre-melanin transporter phase,but has little effect in the later phase.

关 键 词：兰坪乌骨绵羊 黑色素 SLC17A8基因 CRISPR/Cas9 

分 类 号：S813[农业科学—畜牧学]