半夏及其炮制品对SD大鼠的毒性作用  

Toxic Effects of Raw Pinelliae rhizoma and Its Processing Products on SD Rats

作　　者：王淑贞[2] 王琼珺[1,2] 林泽燕[1,2] 刘舜慧[1] 庄苒[1] 许文 WANG Shuzhen;WANG Qiongjun;LIN Zeyan;LIU Shunhui;ZHUANG Ran;XU Wen(Zhangzhou Health Vocational College,Zhangzhou,Fujian 363000;Laboratory of Marine Natural Products and Activity Research,Zhangzhou Health Vocational College,Zhangzhou,Fujian 363000;Centre of Biomedical Research&Development,College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122)

机构地区：[1]漳州卫生职业学院药学院,福建漳州363000 [2]漳州卫生职业学院海洋天然产物与活性研究实验室,福建漳州363000 [3]福建中医药大学药学院生物医药研发中心,福建福州350122

出　　处：《武夷学院学报》2024年第9期20-25,共6页Journal of Wuyi University

基　　金：漳州卫生职业学院优秀人才支持计划(YXRC201704);漳州卫生职业学院科技创新团队支持项目(kjcx-08)。

摘　　要：为研究半夏及其炮制品对大鼠心、肝、肾的毒性作用,将SD大鼠随机分为正常组、生半夏组(3 g/(kg·d))、法半夏组(3 g/(kg·d))、清半夏组(3 g/(kg·d))和姜半夏组(3 g/(kg·d)),连续灌胃21 d后,检测各组血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、尿素氨(BUN)、肌酐(Cr)、肌酸激酶(CK)和乳氨酸脱氢酶(LDH)的变化。用HE染色观察心肌组织病理变化,蛋白免疫印迹(Western blot)法测定心肌组织中TGF-β1/Smads/mTOR信号通路相关蛋白的表达。结果发现生半夏经炮制加工后的法半夏、姜半夏和清半夏炮制品的毒性较生半夏的毒性减弱,即对心、肝、肾均无毒性影响。可见合理炮制对半夏的心毒性的减毒作用有显著的改善,有助于临床半夏合理用药。SD rats were randomly divided into normal group,raw Pinelliae rhizoma group(3g/(kg·d)),Pinelliae rhizoma praeparatum group(3g/(kg·d)),Pinelliae rhizoma praeparatum cumalumine group(3g/(kg·d)),Pinelliae rhizoma praeparatum cum zingibere ET Alumine group(3g/(kg·d))via gavage for additional 21 days.The levels of serum AST,ALT,BUN,Cr,CK and LDH were detected.Myocardial histopathological changes were observed by HE staining,Western blot assay was used to detect the TGF-β1/Smads/mTOR Signaling Pathway Related Proteins in myocardial tissues.The results suggested that raw Pinelliae rhizoma has certain degree of cardiotoxicity.This study provides the mechanisms of raw PR-induced cardiotoxicity and the toxicity-reducing effect of processing and should help in rational clinical application of Pinelliae rhizoma.

关 键 词：生半夏 炮制半夏 大鼠心脏、肝脏、肾脏毒性 TGF-β1/Smads/mTOR信号通路 

分 类 号：R283[医药卫生—中药学]