两种分化试剂对麻风杆菌的染色效果比较  

Comparison of two differentiation reagents for the staining of leprosy bacilli

作　　者：文肖 李佳峰 李帼敏 彭婉晴 薛汝增 WEN Xiao;LI Jiafeng;LI Guomin;PENG Wanqing;XUE Ruzeng(Dermatology Hospital,Southern Medical University,Guangzhou 510091,China)

机构地区：[1]南方医科大学皮肤病医院,广东广州510091

出　　处：《皮肤性病诊疗学杂志》2024年第10期669-673,共5页Journal of Diagnosis and Therapy on Dermato-venereology

摘　　要：目的比较20%硫酸和酸性酒精作为分化试剂对麻风杆菌的染色效果,为麻风患者选取更好的抗酸染色分化试剂。方法选择61例确诊为麻风阳性患者的皮损组织切片,在分化时间为0.5、1.0、1.5 min下,分别采用20%的硫酸和成品试剂盒内酸性酒精分化,观察并比较两种方法下连续切片菌落量以及汗腺附近红色异染颗粒的变化。结果在分化时间0.5、1.0、1.5 min下,同一患者蜡块的连续切片中,20%硫酸均比成品试剂盒内酸性酒精染出更多的菌落(P值分别为0.038、0.012、0.017),并更容易染出汗腺异染小颗粒(均P<0.001)。结论在麻风杆菌染色检测中,使用20%硫酸分化比成品试剂盒内酸性酒精分化的菌落量表达效果更显著。Objective To compare the effects of 20%sulfuric acid and acidic alcohol for the staining of leprosy bacilli in order to choose a better acid fast staining reagent for leprosy patients.Methods Sixty-one sections of the skin from leprosy patients were differentiated with either 20%sulfuric acid or acidic alcohol for 0.5,1 and 1.5 minutes,respectively.The changes in bacterial colony count and red heterochromatic particles around the sweat glands were compared on the sections of the same block between these two methods.Results More bacterial colonies were observed using 20%sulfuric acid than acidic alcohol for all times(P=0.038,0.012,0.017,respectively).Differentiation with 20%sulfuric acid made it easier to observe heterochromatic particles in the sweat glands(P<0.001).Conclusion In the staining of leprosy bacillus,20%sulfuric acid is better than acid alcohol to visualize bacterial colonies.

关 键 词：麻风杆菌 抗酸染色 皮肤组织病理学 20%硫酸 分化试剂 

分 类 号：R755[医药卫生—皮肤病学与性病学] R446.5[医药卫生—临床医学]