基于rbcL叶绿体序列解析金线兰及其近缘种亲缘关系  

Phylogenetic Analysis between Anoectochilus roxborghil and Its Related Species Based on rbcL Chloroplast Sequence

作　　者：叶炜 颜沛沛 王培育 李尊文 吴铭俊 李汉生 林敏水 江金兰 Ye Wei;Yan Peipei;Wang Peiyu;Li Zunwen;Wu Mingjun;Li Hansheng;Lin Minshui;Jiang Jinlan(Sanming Academy of Agricultural Sciences/Fujian Key Laboratory of Crop Genetic Improvement and Innovative Utilization for Mountainous Areas,Sanming,Fujian 365509,China;Shaxian Agriculture and Rural Bureau,Sanming,Fujian 365509,China;Sanming University,Sanming,Fujian 365504,China;Yongan Forestry Bureau,Sanming,Fujian 366038,China)

机构地区：[1]三明市农业科学研究院/福建省(山区)作物遗传改良与创新利用重点实验室,福建三明365509 [2]沙县区农业农村局,福建三明365509 [3]三明学院,福建三明365504 [4]永安市林业局,福建三明366038

出　　处：《东南园艺》2024年第5期341-349,共9页Southeast Horticulture

基　　金：福建省科技计划项目(2023S0018、2023N0047、2024N5008);福建省省级财政林业专项资金(闽财资环指[2020]10号);三明市科技计划项目(2023-N-6、2023-N-19)。

摘　　要：【目的】应用rbcL叶绿体序列解析金线兰及其近缘种亲缘关系。【方法】以血叶兰属及开唇兰属rbcL叶绿体序列设计引物,扩增福建主栽金线兰及其近缘种的DNA序列,并进行系统进化树及序列分析。【结果】从7种金线兰及其近缘种中获得1221 bp rbcL序列,编码406个氨基酸。对来自19种23条金线兰及近缘种rbcL序列的进化树及序列分析结果表明,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰有较近的遗传距离,其次为台湾银线兰,血叶兰与开唇兰属存在较大的遗传距离。23条序列共存在24个多态性位点,及109个变异位点,定义14个单倍型;其中,金线兰与峨眉金线兰、长裂片金线兰及浙江开唇兰共享单倍型,而丽蕾金线兰、滇南开唇兰、滇越金线兰、白线金线兰、钟山金线兰共享单倍型。【结论】rbcL序列可用于金线兰与近缘种的鉴别。【Objective】Based on rbcL chloroplast sequence analysis to determine the phylogenetic relationships between Anoectochilus roxborghil and its related species.【Method】Primers were designed based on the rbcL chloroplast sequences of Ludisia and Anoectochilus genera.Sequences were amplified from the DNA of the main cultivated A.roxborghil and its related species in Fujian,followed by phylogeneticand sequence analysis.【Result】rbcL sequences with 1221 bp and coding 406 amino acids were amplified from 7 A.roxborghil and its related species.Total 19 species and 23 rbcL sequences were using for phylogenetic analysis.Results revealed that A.roxborghil was closely related to A.emeiensis,A.longilobus,and A.zhejiangensis,followed by A.formosanus.And there is a significant genetic distance between Ludisia and Anoectochilus genera.Those 23 sequences generated a total of 24 polymorphic sites and 109 variant sites,defined 14 haplotypes.Among them,A.roxborghil shared same haplotype with A.emeiensis,A.longilobus,and A.zhejiangensis,while A.lylei,A.burmannicus,A.chapaensis,A.albolineatus,A.zhongshanensis shared same haplotype.【Conclusion】rbcL sequences could be used to distinguish between A.roxborghil and its related species.

关 键 词：开唇兰属 金线兰 RBCL序列 叶绿体 

分 类 号：S567.239[农业科学—中草药栽培] R282[农业科学—作物学]