外源褪黑素对芥菜抽薹开花的生理效应及相关基因表达分析  

Analyses of Exogenous Melatonin on Physiological Effects and Gene Expression Associated with Brassica juncea Bolting and Flowering

作　　者：刘炼 李景飞 马关鹏 王志敏[1] 魏大勇 汤青林[1] Liu Lian;Li Jingfei;Ma Guanpeng;Wang Zhimin;Wei Dayong;Tang Qinglin(College of Horticulture and Landscape Architecture,Southwest University,Beibei,Chongqing 400715,China;Horticulture Institute,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550009,China)

机构地区：[1]西南大学园艺园林学院,重庆北碚400715 [2]贵州省农业科学院园艺研究所,贵州贵阳550009

出　　处：《东南园艺》2024年第5期386-395,共10页Southeast Horticulture

基　　金：国家自然科学基金项目(32172547);重庆市农业科技现代先行区县涪陵区项目“芥菜(茎瘤芥)现代生物技术育种”;西南大学涪陵研究院共建项目(FLYJY202401)。

摘　　要：【目的】筛选延迟芥菜抽薹开花的外源褪黑素处理浓度,揭示其生理效应,解析褪黑素调节抽薹相关基因的表达特性,为芥菜抑制抽薹及新品种选育提供理论依据。【方法】以芥菜为研究对象,在苗期喷施不同浓度外源褪黑素,测定植株抽薹开花时间和不同时期生理指标,定量PCR分析抽薹相关基因的表达情况。【结果】采用不同浓度外源褪黑素(0.02、0.2、0.5、1和2 mM)喷施芥菜,其中2 mM褪黑素可延迟抽薹23 d,抽薹时间显著晚于对照(81.25 d)。褪黑素处理后,在芥菜生育时期为49 d、59 d未抽薹期以及109 d和114 d抽薹期植株中,过氧化物酶POD、超氧化物歧化酶SOD活性和可溶性蛋白含量均显著增加,最高可分别比对照增加25.56%、26.51%和24.03%。过氧化氢酶CAT活性和可溶性糖含量在104 d和114 d抽薹期显著增加;丙二醛MDA含量在抽薹期显著降低。褪黑素处理后,芥菜生育时期为39 d、109 d和114 d植株中SOC1、AGL24和PAT2基因表达水平显著降低,PAT15基因的表达显著增加;PAT24基因表达在生育时期为49 d和59 d未抽薹期显著增加,在抽薹期又显著降低。【结论】高浓度外源褪黑素处理可显著延迟芥菜抽薹,增加未抽薹和抽薹植株中POD、SOD和可溶性蛋白含量;褪黑素对SOC1、AGL24、PAT2、PAT15和PAT24基因的表达水平均有影响。【Objective】To screen the concentration of exogenous melatonin to delay bolting and flowering,reveal its physiological effects,analyze the expression characteristics of genes related to bolting,and provide theoretical basis for bolting tolerance production and new variety breeding in Brassica juncea.【Method】The bolting time and many physiological indexes were determined after spraying different concentrations of melatonin at seedling stage,and the relative expression levels of bolting-related genes were analyzed via qRT-PCR in Brassica juncea.【Result】Different concentrations of exogenous melatonin(0.02,0.2,0.5,1 and 2 mM)were used to treat mustard in this study.The bolting and flowering time of Brassica juncea was delayed 23 days by 2 mM exogenous melatonin,which was significantly later than that of the control.The activities of peroxidase(POD),superoxide dismutase(SOD)and the contents of soluble protein were remarkably increased at the 49 d and 59 d growth stages of non-bolting,and the 109 d and 114 d of bolting plants treated with melatonin,and their highest increments were 25.56%,26.51%and 24.03%,respectively.However,the contents of catalase(CAT)and soluble sugar increased significantly at the 104 d and 114 d stages of bolting plants.And the content of malondialdehyde(MDA)decreased significantly at the bolting stage.After melatonin treatment,the expression levels of SOC1,AGL24 and PAT2 genes decreased significantly but the expression of PAT15 gene increased significantly at the 39 d non-bolting stage as well as the 109 d and 114 d bolting stages.In contrast,the expression of PAT24 gene increased significantly at the 49 d and 59 d non-bolting stages but decreased obviously at the bolting stage.【Conclusion】Treatment with high concentration of melatonin significantly delayed bolting,increased POD,SOD and soluble protein contents in the non-bolting and bolting plants.Furthermore,the expression levels of SOC1,AGL24,PAT2,PAT15 and PAT24 genes were affected by the exogenous melatonin at both pre-boltin

关 键 词：芥菜 褪黑素 抽薹开花 生理变化 基因表达 

分 类 号：S637.3[农业科学—蔬菜学]