铕修饰硅纳米颗粒可视化检测铜离子  

Europium-modified silicon nanoparticles for visual detection of copper ions

作　　者：王文一[1] 张学文 闫旭焕 杨秀颖 赵晨楠 田佳伟 WANG Wenyi;ZHANG Xuewen;YAN Xuhuan;YANG Xiuying;ZHAO Chennan;TIAN Jiawei(School ofMaterial Science and Engineering,Tiangong University,Tianjin 300387,China)

机构地区：[1]天津工业大学材料科学与工程学院,天津300387

出　　处：《天津工业大学学报》2024年第5期31-37,共7页Journal of Tiangong University

基　　金：天津市自然科学基金资助项目(18JCQNJC72400);天津市高等教育委员会科技发展基金资助项目(2018ZD14)。

摘　　要：为了实现对水中铜离子(Cu^(2+))的检测,设计开发了比率荧光探针铕修饰硅纳米颗粒(Eu-DPA@SiNPs)。在探针中,SiNPs作为参比信号,Eu^(3+)为响应信号,而2,6-吡啶二羧酸(DPA)作为“天线”敏化Eu^(3+)。采用透射电子显微镜(TEM)、傅里叶变换红外光谱(FTIR)、X射线衍射图谱(XRD)、紫外可见吸收光谱(UV-Vis)对探针进行形貌和结构的表征,用荧光光谱测试其检测性能。结果表明:当DPA与Eu^(3+)的摩尔比为0.5∶1时,探针具有最大淬灭效率;探针为均匀分散的球形颗粒,平均直径为26.15 nm;探针中加入Cu^(2+)后,10 s即反应完全,在0.2~13.0μmol/L范围呈现良好的线性关系,检出限(LOD)为0.41μmol/L,远低于美国环境保护署所规定的饮用水中Cu^(2+)含量最大允许限值,并成功将探针应用到实际水样中检测Cu^(2+);进一步制备了荧光检测试纸,实现了对Cu^(2+)的便携、可视化和半定量检测。A ratiometric fluorescent probe based on europium modified silicon nanoparticles(Eu-DPA@SiNPs)was designed and developed for the detection of copper ions(Cu^(2+))in water.In the probe,SiNPs act as the reference signal,Eu^(3+)is the response signal,and 2,6-pyridinedicarboxylic acid(DPA)acts as the"antenna"to sensitize Eu^(3+).The morphology and structure of the probe were characterized by transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),X-ray diffraction spectroscopy(XRD)and UV-Vis absorption spectroscopy,and the detection performance was tested by fluorescence spectroscopy.The results showed that the probe had a maximum quenching efficiency when the molar ratio of DPA to Eu^(3+)was 0.5∶1;the probe was homogeneously dispersed spherical with an average diameter of 26.15 nm;the reaction was complete in 10 s after the addition of Cu^(2+)to the probe,with good linearity in the range of 0.2-13μmol/L.The limit of detection(LOD)was 0.41μmol/L,which was much lower than the Cu^(2+)content in water specified by the U.S.Environmental Protection Agency,and the probe was successfully applied in the detection of Cu^(2+)in real water samples;further the fluorescence detection test strips is prepared,achieving portable and visual semi-quantitative detection of Cu^(2+).

关 键 词：铕修饰 硅纳米颗粒 荧光检测 铜离子 

分 类 号：TP212.2[自动化与计算机技术—检测技术与自动化装置] O657.3[自动化与计算机技术—控制科学与工程]