植物水杨酸/苦杏仁酸/壬二酸共载纳米乳的制备及祛痘功效研究  

Preparation and Efficacy of Plant Salicylic Acid/Mandelic Acid/Azelaic Acid Co-delivery Nanoemulsion

作　　者：黄晓东 罗丹 陈璇[4] 王曦 洪延涵 刘卫[3,4] HUANG Xiaodong;LUO Dan;CHEN Xuan;WANG Xi;Hong Yanhan;LIU Wei(Guangzhou YueHui Cosmetics Co.,Ltd.,Guangzhou 510440,Guangdong,China;Wuhan Bestcarrier Biotechnology Co.,Ltd.,Wuhan 430075,Hubei,China;National Engineering Research Center for Nanomedicine,Huazhong University of Science and Technology,Wuhan 430075,Hubei,China;College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,Hubei,China)

机构地区：[1]广州悦荟化妆品有限公司,广东广州510440 [2]武汉百思凯瑞生物科技有限公司,湖北武汉430075 [3]华中科技大学国家纳米药物工程技术研究中心,湖北武汉430075 [4]华中科技大学生命科学与技术学院,湖北武汉430074

出　　处：《中国美容医学》2024年第11期6-10,共5页Chinese Journal of Aesthetic Medicine

摘　　要：目的:通过纳米载体技术将植物水杨酸、苦杏仁酸及壬二酸等活性成分进行包载,制备植物水杨酸/苦杏仁酸/壬二酸共载纳米乳(植酸素,产品批号:20220623),对其理化性质、体外透皮性能、抑菌活性及祛痘控油功效进行评价。方法:采用纳米电位粒径分析仪测试植酸素的粒径、PDI及电位;采用Franz扩散池法进行离体猪皮的透皮实验;建立痤疮丙酸杆菌和金黄色葡萄球菌活性抑制的实验方法;建立LPS诱导的细胞炎症模型及采用油红O法观察植酸素对SZ95细胞分泌油脂的影响。结果:测得植酸素粒径为(37.5±0.2)nm,PDI为(0.131±0.008),Zeta电位为(-25.1±1.2)mV。体外透皮实验结果表明,活性成分经纳米载体包裹后具有良好的皮肤渗透性及皮肤储留能力;抑菌实验结果表明,植酸素可抑制痤疮丙酸杆菌和金黄色葡萄球菌活性;细胞功效实验结果表明,与游离活性成分比较,植酸素可显著抑制RAW264.7细胞分泌炎症因子,抑制SZ95细胞分泌细胞脂滴。结论:植物水杨酸、苦杏仁酸及壬二酸经纳米载体包裹后得到的植酸素共载纳米乳,具有良好的体外透皮性能、抑菌活性及祛痘控油功效,在祛痘控油护肤品领域有良好的应用前景。Objective The active ingredients such as plant salicylic acid,mandelic acid and azelaic acid were encapsulated by nanocarrier technology to prepare plant salicylic acid/mandelic acid/azelaic acid co-delivery nanoemulsion(SMA,Batch number:20220623),and evaluate its physicochemical properties,in vitro transdermal properties,antibacterial activity and acne and oil control efficacy.Methods The particle size,PDI and potential of phytochemicals were measured by nanopotential particle size analyzer.Transdermal experiments on isolated porcine skin were conducted by Franz diffusion cell method.Experimental methods for the inhibition of the activity of Propionibacterium acnes and Staphylococcus aureus were established.LPS-induced cellular inflammation model and the effect of phytochemicals on the secretion of oil by SZ95 cells were observed by oil red O method.Results The particle size of SMA was(37.5±0.2)nm,the PDI was(0.131±0.008),and the zeta potential was(25.1±1.2)mV as measured by a nanopotential particle size analyzer.The results of in vitro transdermal experiments show that the active ingredients have good skin permeability and skin storage capacity after being wrapped by nano carriers.The results of antibacterial experiments showed that SMA could inhibit the activity of Propionibacterium acnes and Staphylococcus aureus.The results of cell efficacy experiments showed that SMA could significantly inhibit the secretion of inflammatory factors by RAW264.7 cells and the secretion of cytosolic lipid droplets by SZ95 cells compared with free active ingredients.Conclusion The phytatin co-loaded nano-emulsion,which was coated with plant salicylic acid,amygdalic acid and azelaic acid by nano-carrier,has good in vitro transdermal property,antibacterial activity and anti-acne and oil-control effect,and has a good application prospect in the field of anti-acne and oil-control skin care products.

关 键 词：植物水杨酸/苦杏仁酸/壬二酸 共载纳米乳 透皮实验 抑菌活性 祛痘控油 

分 类 号：TQ658[化学工程—精细化工]