PTEN基因稳定敲除DF-1细胞系的构建及验证  

Construction and Confi rmation of A DF1 Cell Line with PTEN Gene Stable Knockout

作　　者：郭佩东 宁红涯 谭磊[2] 刘炜玮 孙英杰[2] 仇旭升[2] 廖瑛[2] 丁铲 宋翠萍[2] GUO Peidong;NING Hongya;TAN Lei;LIU Weiwei;SUN Yingjie;QIU Xusheng;LIAO Ying;DING Chan;SONG Cuiping(College of Animal Sciences(College of Bee Science),Fujian Agriculture and Forestry University,Fujian 350002,China;Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China)

机构地区：[1]福建农林大学动物科学学院(蜂学学院),福建350002 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国动物传染病学报》2024年第5期185-190,共6页Chinese Journal of Animal Infectious Diseases

基　　金：上海市科技兴农重点攻关项目(沪农科创字(2018)第2-7号);上海市科技创新行动计划(19391902900)。

摘　　要：10号染色体上缺失的磷酸酶和张力蛋白同源物(PTEN)功能丧失导致AKT的活化与积累,在肿瘤发生过程中起着关键作用。为了研究PTEN在禽肿瘤及病毒感染中的作用,构建PTEN基因稳定敲除的细胞株。测定PTEN基因稳定敲除细胞系的增殖能力,细胞周期,氧化应激和对细胞凋亡及其对H9N2及NDV病毒复制的影响。结果表明PTEN基因稳定敲除后促进细胞的增殖和细胞ROS上升以及抑制细胞凋亡,并且敲除PTEN促进了NDV与H9N2病毒的复制,这为后续研究PTEN功能奠定了基础。Function deletion of phosphatase and tensin homolog deleted on chromosome 10(PTEN)leads to activation and accumulation of AKT,which plays a key role in tumorigenesis.In order to study the role of PTEN in avian tumors and virus infection,a cell line with stable PTEN gene knockout was constructed in the present study and confi rmed for its proliferation ability,cell cycle,oxidative stress and eff ects on apoptosis and replication of H9N2 and NDV.The results showed that the stable knockout of PTEN gene promoted cell proliferation and cellular antioxidant capacity and inhibited cell apoptosis,and also promoted replication of NDV and H9N2.The results of this study laid the foundation for the follow-up research of PTEN function.

关 键 词：PTEN 细胞增殖 细胞周期 氧化应激 病毒复制 

分 类 号：Q78[生物学—分子生物学]