HPLC法同时测定五味子根皮中五种木脂素的含量  

Simultaneous Determination of Five Lignans in the Root Bark of Schisandra Chinensis by HPLC Method

作　　者：陈美先 牛昱婷 徐浩南 邓翀 王薇[2] 张东东[2] CHEN Meixian;NIU Yuting;XU Haonan;DENG Chong;WANG Wei;ZHANG Dongdong(Changwu County People s Hospital,Shaanxi Xianyang 713600,China;College of Pharmacy,Shaanxi University of Chinese Medicine,Shaanxi Xianyang 712046,China)

机构地区：[1]长武县人民医院,陕西咸阳713600 [2]陕西中医药大学药学院,陕西咸阳712046

出　　处：《陕西中医药大学学报》2024年第6期24-28,共5页Journal of Shaanxi University of Chinese Medicine

基　　金：国家自然科学基金项目(82174111);陕西中医药大学创新团队项目(2023-CXTD-05)。

摘　　要：目的建立HPLC法同时测定五味子根皮中五味子酚、安五脂素、五味子甲素、五味子乙素及五味子丙素的含量。方法采用Intertsil ODS-3色谱柱(4.6×250 mm,5μm);流动相:乙腈(A)-水(B),梯度洗脱(0~28 min,49%→96%A;28~31 min,96%→96%A;31~35min,96%→100%A);流速0.5 mL·min^(-1);检测波长220 nm;柱温30℃;进样量10μL,测定五味子根皮五味子酚、安五脂素、五味子甲素、五味子乙素及五味子丙素含量。结果该色谱条件下,五种木脂素类化学成分具有良好的线性关系,其含量分别为0.70 mg·g^(-1)、6.53 mg·g^(-1)、2.24 mg·g^(-1)、1.75 mg·g^(-1)、1.16 mg·g^(-1);重复性(RSD)分别为2.65%、1.18%、2.94%、1.53%、1.50%;稳定性(RSD)分别为2.50%、1.60%、1.93%、1.69%、1.86%。结论所建立的含量测定方法稳定可行,重复性好,为后续建立五味子根皮质量标准研究提供了依据。Objective To establish an HPLC method for simultaneous determination of schisandrin,sphingolipids,schisandrin A,schisandrin B,and schisandrin C in the root bark of Schisandra chinensis.Methods Intertsil ODS-3 chromatographic column(4.6×250 mm,5μm)was used;Mobile phase:acetonitrile(A)-water(B),gradient elution(0-28 min,49%→96%A;28-31 min,96%→96%A;31-35min,96%→100%A);Flow rate of 0.5 mL/min;Detection wavelength 220 nm;Column temperature 30℃;The injection volume is 10μL,and the contents of schisandrin,aconitine,schisandrin A,schisandrin B,and schisandrin C in the root bark of Schisandra chinensis were determined.Results Under the chromatographic conditions,the five lignans showed a good linear relationship,with contents of 0.70 mg·g^(-1),6.53 mg·g^(-1),2.24 mg·g^(-1),1.75 mg·g^(-1),1.16 mg·g^(-1) respectively;The repeatability(RSD)were 2.65%,1.18%,2.94%,1.53%,and 1.50%respectively;The stability(RSD)were 2.50%,1.60%,1.93%,1.69%,and 1.86%respectively.Conclusion The established content determination method is stable,feasible,and has good reproducibility,providing a basis for the subsequent establishment of quality standards for Schisandra root bark.

关 键 词：高效液相色谱 五味子 木脂素 含量测定 资源 

分 类 号：R284[医药卫生—中药学]