肝癌患者术后肺部感染病原菌及TGF-β1/Smads信号通路蛋白和Th17/Treg水平  

Levels of TGF-β1/Smads signaling pathway protein,Th17/Treg and pathogens from liver cancer patients with posoperative infection pulmonary

作　　者：陈洋伟 廖成磊 夏丰 罗恩[2] 何旭昶 李波 CHEN Yang-wei;LIAO Cheng-lei;XIA Feng;LUO En;HE Xu-chang;LI Bo(Southwest Medical University Affiliated Hospital,Luzhou,Sichuan 646099,China;不详)

机构地区：[1]西南医科大学附属医院肝胆外科,四川泸州646099 [2]成都市第五人民医院肝胆胰外科,四川成都611130

出　　处：《中华医院感染学杂志》2024年第20期3074-3078,共5页Chinese Journal of Nosocomiology

基　　金：四川省科技厅基金资助项目(2022YFS0256)。

摘　　要：目的探讨肝癌患者术后肺部感染病原菌及转化生长因子-β1(TGF-β1)/Smads信号通路蛋白及辅助性T细胞17(Th17)调节性T细胞(Treg)水平。方法回顾性收集2021年9月-2023年9月西南医科大学附属医院收治的117例进行肝癌手术治疗的肝癌患者的临床资料,依据术后肺部感染情况将患者分为感染组(n=31)与未感染组(n=86);分析感染组患者的病原菌,通过酶联免疫吸附试验测定两组血清白细胞介素(IL)-6、IL-1β及TGF-β1水平,流式细胞仪测定外周血Th17及Treg细胞比例,免疫印迹法测定Smad2、Smad3、Smad6、Smad7、TGF-β1蛋白的表达。结果在31例肺部感染患者中,共培养分离病原菌53株,其中革兰阴性菌34株,革兰阳性菌11株,真菌8株;感染组患者血清IL-6、IL-1β、TGF-β1、TGF-β1蛋白、Smad2、Smad3、Th17细胞比例及Th17/Treg分别为(67.43±12.60)pg/ml、(42.56±8.71)μg/L、(301.29±54.13)pg/ml、13.26±2.05、35.21±4.67、42.89±7.91、(4.18±0.89)%及1.69±0.28高于未感染组(P<0.05),而Smad6、Smad7和Treg细胞比例分别为18.53±3.14、7.44±2.06和(2.47±0.35)%均低于未感染组(P<0.05)。结论肝癌患者术后肺部感染会介导炎性因子释放而激活TGF-β1/Smads信号通路,并引起Th17/Treg失衡。OBJECTIVE To investigate the levels of transforming growth factor-β1(TGF-β1)/Smads signaling path-way protein and helper T cell 17(Th17)regulatory T cells(Treg)in patients with postoperative pulmonary infec-tion of hepatocellular carcinoma.METHODS The clinical data of 117 patients with liver cancer who underwent liver cancer surgery in the Affiliated Hospital of Southwest Medical University from Sep.2021 to Sep.2023 were retro-spectively collected.According to the postoperative pulmonary infection,the patients were divided into the infec-tion group(n=31)and the non-infection group(n=86).The pathogenic bacteria of the patients in the infection group were analyzed,serum interleukin(IL)-6,IL-1βand TGF-β1 levels of the two groups were determined by enzyme-linked immunosorbent assay,the proportion of Th17 and Treg cells in peripheral blood was determined by flow cytometry,and the expression of Smad2,Smad3,Smad6,Smad7 and TGF-β1 protein was determined by immunoblotting.RESULTS Among 31 patients with pulmonary infection,a total of 53 strains of pathogenic bacte-ria were isolated and cultured,of which 34 strains of gram-negative bacteria,ll strains of gram-positive bacteria and 8 strains of fungi.Serum IL-6,IL-1β,TGF-β1,TGF-β1,Smad2,and Smad3 levels,the proportion of Th17 cells,and Th17/Treg in the infection group were(67.43±12.60)pg/ml,(42.56±8.71)μg/L,(301.29±54.13)pg/ml,13.26±2.05,35.21±4.67,42.89±7.91,(4.18±0.89)% and 1.69±0.28,respectively,which were higher than those in the non-infection group(P<0.05),while the proportions of Smad6,Smad7 and Treg cells were 18.53±3.14,7.44±2.06 and(2.47±0.35)%,respectively,which were lower than those in the un infected group(P<0.05).CONCLUSION Postoperative pulmonary infection in patients with liver cancer mediated the activation of the TGF-β1/Smads signaling pathway by the release of inflammatory factors,and caused Th17/Treg imbalance.

关 键 词：肝癌 肺部感染 病原菌 转化生长因子-β1/Smads信号通路蛋白 辅助性T细胞17 调节性T细胞 

分 类 号：R563.1[医药卫生—呼吸系统]