噬菌体Phi-X174和白色葡萄球菌气溶胶对紫外线消毒的抵抗力研究  

Study on the resistance of phage Phi-X174 and Staphylococcus albus aerosol aerosols to ultraviolet light disinfection

作　　者：张宝莹[1] 麻翠翠 段弘扬 王妍彦[1] 沈瑾[1] ZHANG Bao-ying;MA Cui-cui;DUAN Hong-yang;WANG Yan-yan;SHEN Jin(National Institute of Environmental Health,Chinese Center for Disease Control and Prevention,Beijing 100021,China;Beijing Center for Disease Control and Prevention)

机构地区：[1]中国疾病预防控制中心环境与健康相关产品安全所,北京100021 [2]北京市疾病预防控制中心

出　　处：《中国消毒学杂志》2024年第10期721-723,726,共4页Chinese Journal of Disinfection

基　　金：首都卫生发展科研专项(首发2022-2G-4232)。

摘　　要：目的比较气溶胶状态下噬菌体Phi-X174和白色葡萄球菌对紫外线的抵抗力,探索噬菌体Phi-X174作为指示微生物用于空气病毒消毒效果评价的可能性。方法检测一种紫外线消毒设备在不同消毒作用时间下对2种微生物的杀灭率,以D值为指标评价2种微生物气溶胶对紫外线的抵抗力大小。结果以浓度为2×10^(8)pfu/mL的噬菌体悬液雾化1.5 mL,空气消毒舱内噬菌体平均浓度为3.59×10^(6)pfu/m^(3),组间误差率为8.13%;以浓度为8×10^(6)cfu/mL的白色葡萄球菌菌液雾化2 mL,空气消毒舱内细菌平均浓度为7.10×10^(5)cfu/m^(3),组间误差率为10.14%。紫外线消毒设备对噬菌体Phi-X174在5、10、15 min时的平均杀灭率为65.30%、86.89%、93.46%;对白色葡萄球菌在10、15、20 min时的平均杀灭率为74.87%、85.21%、94.92%。结论噬菌体Phi-X174的D值在10~15 min,白色葡萄球菌的D值在15~20 min,即在气溶胶状态下,白色葡萄球菌对该紫外线消毒设备的抵抗力高于噬菌体Phi-X174。Objective To compare the resistance of phage Phi-X174 and Staphylococcus albus to ultraviolet light in aerosol state,and to explore the possibility of phage Phi-X174 as an indicator microorganism for the evaluation of the disinfection effect of air viruses.Methods The reproducibility of the killing rates of two microorganisms by a UV disinfection equipment at different disinfection times were tested,and the resistance of two types of microbial aerosols to UV light was evaluated using D value as the indicator.Results 1.5 mL of phage suspension with a concentration of 2×10^(8)pfu/mL was atomized,and the average concentration of phage in the air disinfection chamber was 3.59×10^(6)pfu/m^(3),with an inter-group error rate of 8.13%;2 mL of Staphylococcus albus suspension with a concentration of 8×10^(6)cfu/mL was atomized,and the average concentration of bacteria in the air disinfection chamber was 7.10×10^(5)cfu/m^(3),with an inter-group error rate of 10.14%.The average ki lling rates of phage Phi-X174 by ultraviolet disinfection equipment at 5,10,and 15 min were 65.30%,86.89%,and 93.46%,respectively.The average killing rates of Staphylococcus albus at 10,15,and 20 min were 74.87%,85.21%,and 94.92%,respectively.Conclusion The D value of phage Phi-X174 is between 10 and 15 min,and the D value of Staphylococcus albus is between 15 and 20 min.Staphylococcus albus has a higher resistance to the UV disinfection equipment than phage Phi-X174 in aerosol state.

关 键 词：噬菌体 白色葡萄球菌 气溶胶 紫外线 沉降率 抵抗力 

分 类 号：R187.2[医药卫生—流行病学]