敲低Runx1增强动脉介入化疗对肺癌大鼠肿瘤侵袭、转移的抑制作用  

Knockdown of Runx1 enhances the inhibitory effects of arterial interventional chemotherapy on tumor invasion and metastasis in lung cancer rats

作　　者：张晰尧 王泽鑫[1] 关利君[1] 王志超 薛梦若 ZHANG Xi-yao;WANG Ze-xin;GUAN Li-jun;WANG Zhi-chao;XUE Meng-ruo(Department of Interventional Radiology,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010050,Inner Mongolia Autonomous Region,China)

机构地区：[1]内蒙古医科大学附属医院介入放射科,内蒙古自治区呼和浩特010050

出　　处：《中国临床药理学杂志》2024年第20期3008-3012,共5页The Chinese Journal of Clinical Pharmacology

摘　　要：目的探讨抑制Runt相关转录因子1(Runx1)表达在动脉介入化疗治疗肺癌大鼠中的作用。方法将A549细胞随机分为对照组细胞(正常培养的细胞)、si-NC组(转染si-NC质粒)、si-Runx1组(转染si-Runx1质粒)。用细胞计数试剂盒-8(CCK-8)实验检测细胞增殖情况,用蛋白质印迹法检测蛋白相对表达水平。将大鼠随机分为模型组(构建肺癌移植瘤大鼠)、sh-Runx1组(敲低Runx1表达)、OXA arterial组(单独动脉介入化疗)、sh-Runx1+OXA组(敲低Runx1+静脉化疗)、sh-Runx1+OXA arterial组(敲低Runx1+动脉介入化疗),连续治疗3周。测量肿瘤体积,用原位末端转移酶标记法(Tunel)实验检测肿瘤细胞凋亡情况,用蛋白质印迹法检测迁移、侵袭相关蛋白表达情况。结果对照组、si-NC、si-Runx1组细胞存活率分别为(100.00±5.13)%、(99.56±3.44)%和(60.96±7.00)%,Runx1蛋白相对表达水平分别为0.84±0.06、0.85±0.06、0.20±0.03。si-Runx1组分别与对照组、si-NC比较,细胞存活率及Runx1蛋白相对表达水平均显著下降(均P<0.05)。模型组、sh-Runx1组、OXA arterial组、sh-Runx1+OXA组、sh-Runx1+OXA arterial组大鼠末次治疗后肿瘤体积分别为(1069.58±121.79)、(819.30±6.98)、(639.34±66.64)、(486.91±29.88)和(416.57±21.58)mm^(3),细胞凋亡率分别为(4.32±0.36)%、(13.95±1.22)%、(15.46±1.14)%、(23.71±2.01)%和(31.16±3.04)%,E-cadherin蛋白相对表达水平分别为0.31±0.05、0.61±0.07、0.67±0.09、0.92±0.07和1.23±0.13。sh-Runx1组、OXA arterial组、sh-Runx1+OXA组、sh-Runx1+OXA arterial组的上述指标分别与模型组比较,在统计学上差异均有统计学意义(均P<0.05);sh-Runx1+OXA arterial组的上述指标分别与sh-Runx1组、OXA arterial组、sh-Runx1+OXA组比较,在统计学上差异均有统计学意义(均P<0.05)。结论抑制Runx1可增强动脉介入化疗对肺癌大鼠凋亡的诱导作用及细胞转移的抑制作用。Objective To investigate the role of inhibition of Runtassociated transcription factor 1(Runx1)expression in arterial interventional chemotherapy for lung cancer in rats.Methods A549cells were randomly divided into control group(normal cultured cells),si-NC group(transfected with si-NC plasmid),si-Runx1 group(transfected with si-Runx1 plasmid).Cell proliferation was detected by cell counting kit-8(CCK-8)assay,and the relative expression level of protein was detected by Western blotting.Rats were randomly divided into model group(constructed lung cancer transplanted tumor rats),sh-Runx1 group(knockdown Runx1expression),ONA arterial group(single arterial interventional chemotherapy),sh-Runx1+OX A group(knockdown Runx1+intravenous chemotherapy),sh-Runx1+OXA arterial group(knockdown Rurx1+arterial interventional chemotherapy).After continuous treatment for 3 weeks,tumor volume and weight were measured,TdT mediated dUDP nick end labeling(Tunel)assay was used to detect tumor apoptosis,and Western blot assay was used to detect the expression of migration and invasion-related proteins.Results The survival rates of A549 cells in the control group,si-NC group and si-Runx1 group were(100.00±5.13)%,(99.56±3.44)%and(60.96±7.00)%,respectively;the expression levels of Runx1 protein were 0.84±0.06,0.85±0.06 and 0.20±0.03,respectively.Compared with the control group and si-NC group,the cell survival rate and Runx1 protein expression level in the si-Runx1 group were significantly decreased(all P<0.05).The tumor volume of the model group,sh-Runx1group,OXA arterial group,sh-Runx1+OXA group and sh-Runx1+OXA arterial group after the last treatment were(1069.58±121.79),(819.30±6.98),(639.34±66.64),(486.91±29.88),(416.57±21.58)mm^(3),respectively;the apoptosis rates were(4.32±0.36)%,(13.95±1.22)%,(15.46±1.14)%,(23.71±2.01)%,(31.16±3.04)%,respectively;the expression levels of E-cadherin protein were 0.31±0.05,0.61±0.07,0.67±0.09,0.92±0.07,1.23±0.13,respectively:The above indexes of sh-Runx1 group,OXA arterial group,s

关 键 词：肺癌 动脉介入化疗 Runt相关转录因子1 协同作用 侵袭和转移 

分 类 号：R979.1[医药卫生—药品]