Simulated Microgravity can Promote the Apoptosis and Change Inflammatory State of Kupffer Cells  

作　　者：Jun Ge Fei Liu Hongyun Nie Yuan Yue Kaige Liu Haiguan Lin Hao Li Tao Zhang Hongfeng Yan Bingxin Xu Hongwei Sun Jianwu Yang Shaoyan Si Jinlian Zhou Yan Cui 

机构地区：[1]Clinical laboratory,the Ninth Medical Center of the PLA General Hospital,Beijing 100101,China [2]Department of General Surgery,Fuyang Affiliated Hospital of Anhui Medical University,Fuyang 236000,Anhui,China [3]Department of General Surgery,the 306th Hospital of PLA-Peking University Teaching Hospital,Beijing 100101,China [4]Department of Disease Control and Prevention,the Ninth Medical Center of PLA General Hospital,Beijing 100101,China [5]Department of General Surgery,PLA Medical College,Beijing 100101,China [6]Department of General Surgery,the Ninth Medical Center of PLA General Hospital,Beijing 100101,China [7]Special Medical Laboratory,the Ninth Medical Center of PLA General Hospital,Beijing 100101,China [8]Department of Pathology,the Ninth Medical Center of PLA General Hospital,Beijing 100101,China

出　　处：《Biomedical and Environmental Sciences》2024年第10期1117-1127,共11页生物医学与环境科学（英文版）

摘　　要：Objective In this study,we analyzed the transcriptome sequences of Kupffer cells exposed to simulated microgravity for 3 d and conducted biological experiments to determine how microgravity initiates apoptosis in Kupffer cells.Methods Rotary cell culture system was used to construct a simulated microgravity model.GO and KEGG analyses were conducted using the DAVID database.GSEA was performed using the R language.The STRING database was used to conduct PPI analysis.qPCR was used to measure the IL1B,TNFA,CASP3,CASP9,and BCL2L11 mRNA expressions.Western Blotting was performed to detect the level of proteins CASP3 and CASP 9.Flow cytometry was used to detect apoptosis and mitochondrial membrane cells.Transmission electron microscopy was used to detect changes in the ultrastructure of Kupffer cells.Results Transcriptome Sequencing indicated that simulated microgravity affected apoptosis and the inflammatory state of Kupffer cells.Simulated microgravity improved the CASP3,CASP9,and BCL2L11 expressions in Kupffer cells.Annexin-V/PI and JC-1 assays showed that simulated microgravity promoted apoptosis in Kupffer cells.Simulated microgravity causes M1 polarization in Kupffer cells.Conclusion Our study found that simulated microgravity facilitated the apoptosis of Kupffer cells through the mitochondrial pathway and activated Kupffer cells into M1 polarization,which can secrete TNFA to promote apoptosis.

关 键 词：MICROGRAVITY APOPTOSIS Kupffer cell POLARIZATION 

分 类 号：R852.22[医药卫生—航空、航天与航海医学]