黄香楝提取物对UVB致HaCaT细胞光老化损伤的保护作用  

Protective effects of Thanaka extract against skin photoaging damage of HaCaT cells caused by UVB

作　　者：徐宛钰 章一新[1] XU Wanyu;ZHANG Yixin(Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市200011

出　　处：《组织工程与重建外科》2024年第5期516-521,共6页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：上海市科学技术委员会临床医学研究中心项目(22MC1940300)。

摘　　要：目的探究黄香楝提取物对中波紫外线(紫外线B,UVB)致皮肤光老化损伤的保护作用。方法使用0~25μg/mL黄香楝提取物及30 mJ/cm^(2)UVB处理人永生化表皮细胞(HaCaT),CCK-8法检测黄香楝提取物对UVB辐照后HaCaT细胞增殖活力的影响。低浓度(3μg/mL)、中浓度(6μg/mL)、高浓度(12μg/mL)黄香楝提取物及30 mJ/cm^(2)UVB处理HaCaT细胞,DCFH-DA荧光探针检测细胞内活性氧(ROS)含量;超氧化物歧化酶(SOD)检测抗氧化活性;脂质过氧化(MDA)检测氧化损伤水平;JC-1荧光探针检测线粒体膜电位变化。结果0~25μg/mL各浓度黄香楝提取物处理对细胞增殖活性均无显著影响;与UVB组相比,经12μg/mL黄香楝提取物处理后,HaCaT细胞增殖活性上升(P<0.05)。进一步实验显示,与UVB组相比,中、高浓度黄香楝提取物组ROS含量下降(P<0.05),SOD活性上升(P<0.05),低、中、高浓度黄香楝提取物组MDA水平下降(P<0.05),线粒体膜电位回升。结论黄香楝提取物可增强经UVB辐照后HaCaT细胞的增殖活性及抗氧化水平,减少UVB产生的氧化应激损伤,其潜在机制可能与线粒体功能相关。Objective To investigate the protective effect of Thanaka extract against UVB-induced photoaging damage in skin.Methods HaCaT cells were treated with 0-25μg/mL Thanaka extract and 30 mJ/cm^(2) UVB.CCK-8 method was used to observe the effect of Thanaka extract and its effect on the viability of HaCaT cells after UVB irradiation.HaCaT cells were treated with low(3μg/mL),medium(6μg/mL)and high(12μg/mL)concentrations of Thanaka extract and 30 mJ/cm^(2) UVB.DCFH-DA fluorescent probe was used to detect intracellular reactive oxygen species(ROS)content;JC-1 fluorescent probe was used to detect mitochondrial membrane potential;Superoxide dismutase(SOD)and malondialdehyde(MDA)were used to detect the levels of antioxidant and oxidative damage.Results The treatment of 0-25μg/mL of Thanaka extract at all concentrations had no significant effect on cell viability.The viability of HaCaT cells was increased after treatment with 12μg/mL of Thanaka extract compared with that of the UVB group(P<0.05).Further experiments showed that ROS content decreased(P<0.05),SOD activity increased(P<0.05)in the medium and high concentration of Thanaka extract groups;MDA level decreased(P<0.05)and mitochondrial membrane potential rebounded in the low,medium,and high concentration of Thanaka extract groups compared with the UVB group.Conclusion Thanaka extract can promote the viability and antioxidant level of HaCaT cells after UVB irradiation,and reduce the oxidative stress damage produced by UVB,and its underlying mechanism may be related to mitochondrial function.

关 键 词：黄香楝提取物 中波紫外线 光老化 活性氧 线粒体 

分 类 号：R751.05[医药卫生—皮肤病学与性病学]