柠檬醛通过EGFR信号通路抑制NCI-H1975细胞增殖  

Citral Inhibits Proliferation of NCI-H1975 Cells via the EGFR Signaling Pathway

作　　者：朱威巍 聂妍 翁大志 李艳丽 王宣军[2] 黄业伟[2] 范源洪 ZHU Weiwei;NIE Yan;WENG Dazhi;LI Yanli;WANG Xuanjun;HUANG Yewei;FAN Yuanhong(College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Key Laboratory of Pu'er Tea Science,Ministry of Education,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Yunnan Plateau Characteristic Agricultural Industry Research Institute,Kunming 650201,Yunnan,China)

机构地区：[1]云南农业大学食品科学技术学院,云南昆明650201 [2]云南农业大学普洱茶学教育部重点实验室,云南昆明650201 [3]云南省高原特色农业产业研究院,云南昆明650201

出　　处：《食品研究与开发》2024年第21期63-67,共5页Food Research and Development

基　　金：云南绿色食品国际合作研究中心重大科技专项(2019ZG00903)。

摘　　要：该文探究柠檬醛是否通过表皮生长因子受体(epidermal growth factor receptor,EGFR)信号通路诱导非小细胞肺癌(non-small cell lung cancer,NSCLC)系中NCI-H1975细胞增殖。主要采用噻唑蓝(methyl thiazolyl tetrazolium,MTT)法检测细胞毒性、流式细胞术检测细胞凋亡率、分子对接探究柠檬醛与EGFR蛋白结合位点,通过蛋白质免疫印迹法(Western Blot)检测柠檬醛对EGFR及其下游细胞外调节蛋白激酶(extracellular regulated protein kinases,ERK)蛋白表达的影响。研究结果显示柠檬醛在NCI-H1975细胞系上IC50值为114.5μmol/L,且100μmol/L的柠檬醛已对NCIH1975细胞的增殖产生显著抑制作用(P<0.05),呈浓度依赖性抑制细胞增殖;流式结果显示柠檬醛可增大细胞凋亡率,促进细胞凋亡;Western Blot检测结果表明,柠檬醛在100μmol/L时对EGFR/ERK蛋白磷酸化表达有显著抑制作用(P<0.05)。综上,柠檬醛可抑制NCI-H1975细胞增殖并能促进凋亡,通过抑制EGFR信号通路传导进而发挥作用。The effect of citral on the proliferation of the non-small cell lung cancer(NSCLC)cell line NCIH1975 via the epidermal growth factor receptor(EGFR)signaling pathway was studied.The methyl thiazolyl tetrazolium(MTT)assay and flow cytometry were employed to detect the cytotoxicity of citral and the apoptosis of cells,respectively.The binding site of citral to EGFR was studied by molecular docking.The effect of citral on the protein expression of EGFR and its downstream extracellular regulated protein kinases(ERK)was detected by protein immunoblotting(Westerm Blot).The results showed that the half maximal inhibitory concentration(IC50)of citral on NCI-H1975 cells was 114.5μmol/L,and citral at 100μmol/L inhibited the proliferation of NCI-H1975 cells(P<0.05).Moreover,the inhibition was in a concentration-dependent manner.Citral promoted the apoptosis of cells.Citral at 100μmol/L inhibited EGFR/ERK phosphorylation(P<0.05).In conclusion,citral inhibited the proliferation and promoted the apoptosis of NCI-H1975 cells by inhibiting the EGFR signaling pathway.

关 键 词：柠檬醛 NCI-H1975 表皮生长因子受体 细胞增殖 细胞凋亡 

分 类 号：TQ460.1[化学工程—制药化工]