ICP-MS法结合化学计量学分析甘松不同药用部位无机元素的差异  

Analysis of Inorganic Elements in Different Medicinal Parts of Nardostachys jatamansi DC.by ICP-MS Combined with Chemometrics

作　　者：丰日落 土比林场 额其小里 罗江 李文兵 刘圆 钟海蓉 李莹 FENG Riluo;Tubilinchang;Eqixiaoli;LUO Jiang;LI Wenbing;LIU Yuan;ZHONG Hairong;LI Ying(College of Pharmacy,Southwest Minzu University,Chengdu 610225,China;Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory/Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People s Republic of China,Southwest Minzu University,Chengdu 610225,China;Sichuan College of Traditional Chinese Medicine,Mianyang Sichuan 621000,China)

机构地区：[1]西南民族大学药学院,成都610225 [2]青藏高原民族药用资源保护与利用国家民委重点实验室/四川省羌彝药用资源保护与利用技术工程实验室,成都610225 [3]四川中医药高等专科学校,四川绵阳621000

出　　处：《西南大学学报（自然科学版）》2024年第11期81-99,共19页Journal of Southwest University(Natural Science Edition)

基　　金：国家重点研发计划项目(2018YFC1708005);四川省自然科学基金项目(2022NSFSC1588);西南民族大学中央高校基本科研业务费专项(2023NYXXS075)。

摘　　要：建立电感耦合等离子体质谱法(Inductively Coupled Plasma Mass Spectrometry,ICP-MS)同时测定甘松不同药用部位中25种无机元素.通过ICP-MS对甘松样品中的25个无机元素进行定量分析,并运用主成分分析(Principal Component Analysis,PCA)和正交偏最小二乘判别分析(Orthogonal Partial Least Squares Discriminant Analysis,OPLS-DA)方法综合分析评估甘松不同药用部位的无机元素分布特征.结果显示:常量元素K,Ca,Na和Mg在甘松各部位中质量分数均较高,叶中的K,Ca质量分数分别为根及根茎中的1.57和1.19倍.微量元素及其他元素在根及根茎的丰富度高于叶部.OPLS-DA分析表明:Al,K,Fe,Na,Ca为甘松的特征元素,根及根茎中Na,Al,Fe的质量分数分别为叶部的3.15,2.71和2.48倍.青海省久治县索呼日麻乡、黄南州河南县赛尔龙乡、果洛州久治县智青松多镇,四川省阿坝州红原县邛溪镇、麦洼乡、阿木乡、安曲镇、若尔盖县班佑乡以及甘肃省甘南州玛曲县欧拉镇的根及根茎样品中As元素超出现行《药用植物及制剂进出口绿色行业标准》限度.甘松不同药用部位独特的组成特征可为甘松药材的质量控制及安全性评价提供依据.To establish a method for simultaneous determination of 25 inorganic elements in different medicinal parts of Nardostachys jatamansi DC.by Inductively Coupled Plasma Mass Spectrometry(ICP-MS),ICP-MS was used to determine 25 inorganic elements in N.jatamansi DC.samples for quantitative analysis.Principal Component Analysis(PCA) and Orthogonal Partial Least Squares Discriminant Analysis(OPLS-DA) were used to comprehensively analyze and evaluate the distribution characteristics of inorganic elements in different medicinal parts of N.jatamansi DC.plants.The contents of major elements K,Ca,Na and Mg in all parts of N.jatamansi DC.were higher than other inorganic elements,and the contents of K and Ca in leaves were 1.57 and 1.19 times higher than those in roots and rhizomes,respectively.The abundance of trace elements and other elements in roots and rhizomes was higher than that in leaves.OPLS-DA analysis showed that Al,K,Fe,Na and Ca were the characteristic elements of N.jatamansi DC..The contents of Na,Al and Fe in roots and rhizomes were significantly higher than those in leaves by 3.15,2.71 and 2.48 times,respectively.The As in root and rhizome samples of Suohurima Town in Jiuzhi County of Qinghai Province,Saierlong Town in Henan County of Huangnan Prefecture,Zhiqingsongduo Town in Jiuzhi County of Guoluo Prefecture,Qiongxi Town,Maiwa Town,Amu Town,Anqu Town in Hongyuan County of Aba Prefecture,Sichuan Province,Banyou Town in Ruoergai County and Oula Town in Maqu County of Gannan Prefecture,Gansu Province exceeded the limit of current ‘Green industry standard for import and export of medicinal plants and preparations'.The unique composition characteristics of different medicinal parts of Nardostachyos Radix et Rhizome can provide a basis for quality control and safety evaluation of Nardostachyos Radix et Rhizome.This study established a method for accurate and efficient analysis and evaluation of inorganic elements in different medicinal parts of N.jatamansi DC..The method is accurate and efficient,and provides a

关 键 词：甘松 不同药用部位 无机元素 电感耦合等离子体质谱法 特征元素 

分 类 号：S567.23[农业科学—中草药栽培]