丝瓜子房培养类胚体发生途径诱导研究  

Induction Study on the Pathway of Embryoid Formation in Ovary Culture of Luffa ssp.

作　　者：颜乐龙 黄耀 倪维晨 冯翠 刘慧颖 吉茹 苏小俊[3] 钱春桃[4] YAN Le-long;HUANG Yao;NI Wei-chen(Nanjing Agricultural University(Changshu)New Rural Development Research Institute Co.,Ltd.,Suzhou,Jiangsu 215500)

机构地区：[1]南农大(常熟)新农村发展研究院有限公司,江苏苏州215500 [2]江苏省农业科学院泰州农科所,江苏泰州225300 [3]江苏省农业科学院,江苏南京210095 [4]南京农业大学葫芦科作物遗传与种质创新实验室,江苏南京210095

出　　处：《安徽农业科学》2024年第21期44-49,62,共7页Journal of Anhui Agricultural Sciences

基　　金：江苏省自主创新项目(CX202204);常熟市科技项目(CN20-2204)。

摘　　要：[目的]以丝瓜(Luffa ssp.)品种“徐绿一号”的未授粉子房为试验材料,分析不同因素对类胚体形成的影响。[方法]设置L 16(215)正交试验分析子房发育阶段、4℃预冷处理、35℃黑暗热激处理、TDZ、6-BA、ABA、NAA、2,4-D对丝瓜离体子房培养获得类胚体的影响。[结果]不同因素对子房片的发育过程具有不同的效果,其中采集当天开放的雌花、4℃预冷处理、在MS培养基中添加0.04 mg/L TDZ对子房片的类胚体形成具有促进作用,添加0.04 mg/L 2,4-D对丝瓜子房类胚体形成具有抑制作用,但可以促进子房片生根;另外预冷处理与TDZ、TDZ与雌花采集时间、4℃预冷和ABA的两两交互作用对丝瓜类胚体的形成也具有显著影响。[结论]有效形成丝瓜子房类胚体途径:当天采集子房+4℃预冷处理+0.04 mg/L TDZ。[Objective]To analyze the effects of different factors on embryoid formation using unpollinated ovaries of Luffa ssp.cultivar‘Xulv No.1’as experimental material.[Method]An L 16(215)orthogonal test was set up to analyze the effects of ovary collection time,4℃cold pretreatment,35℃dark heat shock treatment,TDZ hormone,6-BA hormone,ABA hormone,NAA hormone and 2,4-D hormone on embryoid formation in vitro cultured Luffa ssp.ovaries.[Result]Different factors had different effects on the development process of ovary slices.Among them,collecting female flowers that opened on the same day and adding TDZ hormone to MS medium had a promoting effect on embryoid formation in ovary slices.However,2,4-D hormone had an inhibitory effect on embryoid formation in Luffa ssp.ovaries but could promote rooting of ovary slices.In addition,the interaction between 4℃cold pretreatment and TDZ hormone and between TDZ hormone and female flower collection time also had a significant effect on embryoid formation in Luffa ssp..[Conclusion]The effective way to form embryoids from Luffa ssp.ovaries is:collecting ovaries on day of flowering+4℃cold pretreatment+0.04 mg/L TDZ hormone induction in vitro.

关 键 词：丝瓜 离体子房 类胚体 单倍体 TDZ 

分 类 号：S642.4[农业科学—蔬菜学]