黑桑枝乙酸乙酯部位化学成分及其葡萄糖摄取活性研究  

Chemical constituents from the ethyl acetate fraction of branches of Morus nigra and their glucose uptake activities

作　　者：周永科 张亦婷 高薇 亢诗雯 庞克坚 杨新洲[2] ZHOU Yong-ke;ZHANG Yi-ting;GAO Wei;KANG Shi-wen;PANG Ke-jian;YANG Xin-zhou(Ministry of Education Key Laboratory for Xinjiang Phytomedicine Resource and Utilization,School of Pharmacy,Shihezi University,Shihezi 832003,China;School of Pharmaceutical Sciences,South-Central Minzu University,Wuhan 430074,China)

机构地区：[1]石河子大学药学院,新疆植物药资源利用教育部重点实验室,新疆石河子832003 [2]中南民族大学药学院,湖北武汉430074

出　　处：《中成药》2024年第10期3316-3323,共8页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金资助项目(82160796)。

摘　　要：目的研究黑桑枝乙酸乙酯部位化学成分及其葡萄糖摄取活性。方法提取物采用硅胶、Sephadex LH-20、半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用L6细胞评价葡萄糖摄取活性。结果从中分离得到16个化合物,分别鉴定为(R)-2-(3,4-dihydroxyphenyl)-3,5,9-trihydroxy-8,8-dimethyl-9,10-dihydropyranochromone(1)、moracin C(2)、nigranol C(3)、nigragenon E(4)、8-prenylquercetin(5)、nigrasin I(6)、sinopodophylline B(7)、kuwanon C(8)、gancaonin O(9)、albanin A(10)、6-O-methyl-moracin M(11)、wittifuran W(12)、8-hydroxy-2,2-dimethyl-2 H,5 H-pyrano[3,2-c]chromen-5-one(13)、norartocarpanone(14)、norartocarpetin(15)、nigrasin D(16)。化合物4、13的葡萄糖摄取量分别为空白组的1.97、2.78倍。结论化合物1为新化合物,5、7、9、12、13、15为首次从黑桑中分离得到。化合物4、13可显著促进L6细胞对葡萄糖的摄取,具有较强的降血糖活性。AIM To study the chemical constituents from the ethyl acetate fraction of the branches of Morus nigra L.and their glucose uptake activities.METHODS The extract was isolated and purified by silica gel,Sephadex LH-20 and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.L6 cells were used to evaluate glucose uptake activities.RESULTS Sixteen compounds were isolated and identified as(R)-2-(3,4-dihydroxyphenyl)-3,5,9-trihydroxy-8,8-dimethyl-9,10-dihydropyranochromone(1),moracin C(2),nigranol C(3),nigragenon E(4),8-prenylquercetin(5),nigrasin I(6),sinopodophylline B(7),kuwanon C(8),gancaonin O(9),albanin A(10),6-O-methyl-moracin M(11),wittifuran W(12),8-hydroxy-2,2-dimethyl-2 H,5H-pyrano[3,2-c]chromen-5-one(13),norartocarpanone(14),norartocarpetin(15),nigrasin D(16).The glucose uptake of compounds 4 and 13 was 1.97 times and 2.78 times that of the control group,respectively.CONCLUSION Compounds 1 is a novel natural product,5,7,9,12,13 and 15 are isolated from M.nigra for the first time.Compounds 4 and 13 can significantly promote the glucose uptake in L6 cells,showing strong hypoglycemic activities.

关 键 词：黑桑 乙酸乙酯部位 化学成分 分离鉴定 葡萄糖摄取 

分 类 号：R284.1[医药卫生—中药学]