噪音环境对黄土边坡微生物群落的影响  

Noise affects microbial communities on loess slopes

作　　者：李明凯 赵丽萍 李学珍 杭鑫余 田丽娇 陈莉[1] LI Mingkai;ZHAO Liping;LI Xuezhen;HANG Xinyu;TIAN Lijiao;CHEN Li(Life Science and Engineering College,Northwest Minzu University,Lanzhou 730106,Gansu,China)

机构地区：[1]西北民族大学生命科学与工程学院,甘肃兰州730106

出　　处：《微生物学报》2024年第11期4219-4233,共15页Acta Microbiologica Sinica

基　　金：甘肃省重点研发计划(22YF7FA172)。

摘　　要：【目的】探讨不同噪音下黄土边坡土壤微生物在温度和时间影响下的变化。【方法】通过测定土壤磷酸盐含量变化,确定噪音分贝在70、90、110 dB,温度在-5、15、35℃,噪音时间在2、4、6 h的条件下,土壤微生物群落的变化。随后对土壤微生物进行宏基因组学测序。【结果】门水平上,放线菌门(Actinobacteria)、毛霉菌门(Mucoromycota)、热变形菌门(Thermoproteota)和黏球菌门(Myxococcota)组间存在显著差异(P<0.05)。属水平上,节杆菌属(Arthrobacter)、根孢囊霉属(Rhizophagus)、假节杆菌属(Pseudarthrobacter)、马杜拉放线菌属(Actinomadura)、考克氏菌属(Kocuria)、红色杆形菌属(Rubrobacter)和棒杆菌属(Corynebacterium)组间存在显著差异(P<0.05);种水平上,Rhizophagusirregularis、Actinomadurasp.WMMB499、热带红色杆形菌(Rubrobactertropicus)、Arthrobactersp.PGP41、Arthrobactersp.24S42和Arthrobactercrystallopoietes组间存在显著差异(P<0.05)。【结论】不同噪音环境对门、属、种分类水平下的土壤微生物均具有显著影响。[Objective]To investigate the changes of microbial communities on loess slopes with different noise conditions under the influences of temperature and time.[Methods]Based on the changes in soil phosphate content,the noise was determined at 70,90,and 110 dB.The microbial communities presented variations along the temperature gradients of−5,15,and 35°C and the noise duration of 2,4,and 6 h.Subsequently,metagenomic sequencing was carried out for the soil microbial communities.[Results]At the phylum level,Actinobacteria,Mucoromycota,Thermoproteota,and Myxococcota showed differences in the relative abundance among groups(P<0.05).At the genus level,Arthrobacter,Rhizophagus,Pseudarthrobacter,Actinomadura,Kocuria,Rubrobacter,and Corynebacterium demonstrated different relative abundance among groups(P<0.05).At the species level,there were significant differences in the relative abundance of Rhizophagus irregularis,Actinomadura sp.WMMB 499,Rubrobacter tropicus,Arthrobacter sp.PGP41,Arthrobacter sp.24S42,and Arthrobacter crystallopoietes among groups(P<0.05).[Conclusion]Different noise environments have significant effects on the relative abundance of soil microorganisms at the phylum,genus,and species levels.

关 键 词：黄土边坡 土壤微生物 噪音 温度 宏基因组 

分 类 号：S154.3[农业科学—土壤学]